ARG53933

anti-GCPII / PSMA antibody [GCP-04]

anti-GCPII / PSMA antibody [GCP-04] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Pig,Rat

Cancer antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description

Mouse Monoclonal antibody [GCP-04] recognizes GCPII / PSMA

Tested Reactivity Hu, Ms, Rat, Pig
Tested Application ICC/IF, IHC-P, WB
Specificity The clone GCP-04 recognizes amino acids 100-104 of extracellular domain of denaturated glutamate carboxypeptidase II (PSMA, NAALADase, FOLH1), an approximately 95-110 kDa transmembrane glycoprotein.
Host Mouse
Clonality Monoclonal
Clone GCP-04
Isotype IgG1
Target Name GCPII / PSMA
Antigen Species Human
Immunogen Recombinant fragment of human GCPII (amino acids 44-750) produced in S2 cells.
Conjugation Un-conjugated
Alternate Names FGCP; GCP2; PSM; Glutamate carboxypeptidase II; PSMA; NAALADase I; Membrane glutamate carboxypeptidase; Prostate-specific membrane antigen; FOLH; N-acetylated-alpha-linked acidic dipeptidase I; GCPII; Glutamate carboxypeptidase 2; NAALAdase; NAALAD1; Folate hydrolase 1; EC 3.4.17.21; Folylpoly-gamma-glutamate carboxypeptidase; mGCP; Cell growth-inhibiting gene 27 protein; Pteroylpoly-gamma-glutamate carboxypeptidase

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
IHC-PAssay-dependent
WB1 µg/ml
Application Note WB: Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% NP-40). Incubate 30 min on ice. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control WB: LNCaP

Properties

Form Liquid
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2346 Human FOLH1

GeneID: 397677 Pig FOLH1

GeneID: 53320 Mouse FOLH1

Gene Symbol FOLH1
Gene Full Name folate hydrolase (prostate-specific membrane antigen) 1
Background Glutamate carboxypeptidase II (GCPII), also known as N-acetyl-alpha-linked acidic dipeptidase I (NAALADase I), folate hydrolase (FOLH1), and prostate-specific membrane antigen (PSMA), is an approximately 95-110 kDa type II transmembrane glycoprotein expressed in various tissues. In nervous system GCPII cleaves abundant N-acetylaspartylglutamate, which is released from neurons in a calcium-dependent manner, to N-acetylaspartate and glutamate. As immoderate glutamate concentration is neurotoxic, GCPII contributes to pathological conditions regarding e.g. Alzheimer´s disease, Huntington´s disease, epilepsy, schizophrenia, stroke or neuropathic pain and appears to be an interesting therapeutic target. In jejunum GCPII hydrolyzes pteroylpoly-gamma-glutamate to folate and glutamate, enabling folate to be absorbed by gastrointestinal tract. GCPII, which is present in a number of tissues at low levels, is overexpressed in neovasculature of most solid tumours and is a target enzyme for diagnosis and treatment of prostate cancer. Normal human prostate express more mRNA coding for a cytosolic GCPII form truncated at the N-terminus (PSM´) than mRNA for membrane-bound GCPII, and this ratio is reversed upon malignant transformation.
Function Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression.
Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC. [UniProt]
Research Area Cancer antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 84 kDa
PTM The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.
The N-terminus is blocked.

Images (4) Click the Picture to Zoom In

  • ARG53933 anti-GCPII / PSMA antibody [GCP-04] WB image

    Western blot: 1) 800 pg, 2) 400 pg, and 3) 200 pg of S2 cell lysate stained with ARG53933 anti-GCPII / PSMA antibody [GCP-04].

  • ARG53933 anti-GCPII / PSMA antibody [GCP-04] IHC-P image

    Immunohistochemistry: Human Medulla oblongata section stained with ARG53933 anti-GCPII / PSMA antibody [GCP-04].
    Magnification 40x.

  • ARG53933 anti-GCPII / PSMA antibody [GCP-04] IHC-P image

    Immunohistochemistry: Human prostate section stained with ARG53933 anti-GCPII / PSMA antibody [GCP-04].
    Magnification 400x.

  • ARG53933 anti-GCPII / PSMA antibody [GCP-04] IHC-P image

    Immunohistochemistry: Porcine kidney section stained with ARG53933 anti-GCPII / PSMA antibody [GCP-04].