ARG53932

anti-GCPII / PSMA antibody [GCP-05] (PE)

anti-GCPII / PSMA antibody [GCP-05] (PE) for Flow cytometry and Human

Cancer antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description

PE-conjugated Mouse Monoclonal antibody [GCP-05] recognizes GCPII / PSMA

Tested Reactivity Hu
Tested Application FACS
Specificity The clone GCP-05 recognizes extracellular domain of glutamate carboxypeptidase II (NAALADase, FOLH1, PSMA), an approximately 95-110 kDa transmembrane glycoprotein expressed mainly in tumour neovasculatures, nervous system and jejunum, which is an important prostate tumour marker.
Host Mouse
Clonality Monoclonal
Clone GCP-05
Isotype IgG1
Target Name GCPII / PSMA
Antigen Species Human
Immunogen amino acids 44-750 of human GCPII
Conjugation PE
Alternate Names FGCP; GCP2; PSM; Glutamate carboxypeptidase II; PSMA; NAALADase I; Membrane glutamate carboxypeptidase; Prostate-specific membrane antigen; FOLH; N-acetylated-alpha-linked acidic dipeptidase I; GCPII; Glutamate carboxypeptidase 2; NAALAdase; NAALAD1; Folate hydrolase 1; EC 3.4.17.21; Folylpoly-gamma-glutamate carboxypeptidase; mGCP; Cell growth-inhibiting gene 27 protein; Pteroylpoly-gamma-glutamate carboxypeptidase

Application Instructions

Application Suggestion
Tested Application Dilution
FACS5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Concentration 0.1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2346 Human FOLH1

Swiss-port # Q04609 Human Glutamate carboxypeptidase 2

Gene Symbol FOLH1
Gene Full Name folate hydrolase (prostate-specific membrane antigen) 1
Background Glutamate carboxypeptidase II (GCPII), also known as N-acetyl-alpha-linked acidic dipeptidase I (NAALADase I), folate hydrolase (FOLH1), and prostate-specific membrane antigen (PSMA), is an approximately 95-110 kDa type II transmembrane glycoprotein expressed in various tissues. In nervous system GCPII cleaves abundant N-acetylaspartylglutamate, which is released from neurons in a calcium-dependent manner, to N-acetylaspartate and glutamate. As immoderate glutamate concentration is neurotoxic, GCPII contributes to pathological conditions regarding e.g. Alzheimer´s disease, Huntington´s disease, epilepsy, schizophrenia, stroke or neuropathic pain and appears to be an interesting therapeutic target. In jejunum GCPII hydrolyzes pteroylpoly-gamma-glutamate to folate and glutamate, enabling folate to be absorbed by gastrointestinal tract. GCPII, which is present in a number of tissues at low levels, is overexpressed in neovasculature of most solid tumours and is a target enzyme for diagnosis and treatment of prostate cancer. Normal human prostate express more mRNA coding for a cytosolic GCPII form truncated at the N-terminus (PSM´) than mRNA for membrane-bound GCPII, and this ratio is reversed upon malignant transformation.
Function Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression.
Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC. [UniProt]
Research Area Cancer antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 84 kDa
PTM The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.
The N-terminus is blocked.

Images (1) Click the Picture to Zoom In

  • ARG53932 anti-GCPII / PSMA antibody [GCP-05] (PE) FACS image

    Flow Cytometry: Separation of LNCaP cells (red) from SP2 cells (blue). Cells were stained with ARG53932 anti-GCPII / PSMA antibody [GCP-05] (PE) at 3 µg/ml dilution.

Clone References

Gold nano-popcorn-based targeted diagnosis, nanotherapy treatment, and in situ monitoring of photothermal therapy response of prostate cancer cells using surface-enhanced Raman spectroscopy.

ICC/IF / Human

Lu W et al.
J Am Chem Soc.,  (2010)

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Expression of glutamate carboxypeptidase II in human brain.

Sácha P et al.
Neuroscience.,  (2007)

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Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding.

Barinka C et al.
Eur J Biochem.,  (2004)

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Identification of the N-glycosylation sites on glutamate carboxypeptidase II necessary for proteolytic activity.

Barinka C et al.
Protein Sci.,  (2004)

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