anti-GCPII / PSMA antibody [GCP-05] (PE)
anti-GCPII / PSMA antibody [GCP-05] (PE) for Flow cytometry and Human
Cancer antibody; Metabolism antibody; Signaling Transduction antibody
PE-conjugated Mouse Monoclonal antibody [GCP-05] recognizes GCPII / PSMA
|Specificity||The clone GCP-05 recognizes extracellular domain of glutamate carboxypeptidase II (NAALADase, FOLH1, PSMA), an approximately 95-110 kDa transmembrane glycoprotein expressed mainly in tumour neovasculatures, nervous system and jejunum, which is an important prostate tumour marker.|
|Target Name||GCPII / PSMA|
|Immunogen||amino acids 44-750 of human GCPII|
|Alternate Names||FGCP; GCP2; PSM; Glutamate carboxypeptidase II; PSMA; NAALADase I; Membrane glutamate carboxypeptidase; Prostate-specific membrane antigen; FOLH; N-acetylated-alpha-linked acidic dipeptidase I; GCPII; Glutamate carboxypeptidase 2; NAALAdase; NAALAD1; Folate hydrolase 1; EC 184.108.40.206; Folylpoly-gamma-glutamate carboxypeptidase; mGCP; Cell growth-inhibiting gene 27 protein; Pteroylpoly-gamma-glutamate carboxypeptidase|
|Application Note||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|Purification Note||The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.|
|Buffer||PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA|
|Preservative||15 mM Sodium azide|
|Stabilizer||0.2% (w/v) high-grade protease free BSA|
|Storage Instruction||Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||folate hydrolase (prostate-specific membrane antigen) 1|
|Background||Glutamate carboxypeptidase II (GCPII), also known as N-acetyl-alpha-linked acidic dipeptidase I (NAALADase I), folate hydrolase (FOLH1), and prostate-specific membrane antigen (PSMA), is an approximately 95-110 kDa type II transmembrane glycoprotein expressed in various tissues. In nervous system GCPII cleaves abundant N-acetylaspartylglutamate, which is released from neurons in a calcium-dependent manner, to N-acetylaspartate and glutamate. As immoderate glutamate concentration is neurotoxic, GCPII contributes to pathological conditions regarding e.g. Alzheimer´s disease, Huntington´s disease, epilepsy, schizophrenia, stroke or neuropathic pain and appears to be an interesting therapeutic target. In jejunum GCPII hydrolyzes pteroylpoly-gamma-glutamate to folate and glutamate, enabling folate to be absorbed by gastrointestinal tract. GCPII, which is present in a number of tissues at low levels, is overexpressed in neovasculature of most solid tumours and is a target enzyme for diagnosis and treatment of prostate cancer. Normal human prostate express more mRNA coding for a cytosolic GCPII form truncated at the N-terminus (PSM´) than mRNA for membrane-bound GCPII, and this ratio is reversed upon malignant transformation.|
|Function||Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression.
Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC. [UniProt]
|Research Area||Cancer antibody; Metabolism antibody; Signaling Transduction antibody|
|Calculated MW||84 kDa|
|PTM||The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.
The N-terminus is blocked.
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