ARG52312

anti-GFAP antibody

anti-GFAP antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunohistochemistry (PFA perfusion fixed frozen sections),Western blot and Cat,Dog,Human,Mouse,Rat

Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody
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Overview

Product Description

Rabbit Polyclonal antibody recognizes Glial Fibrillary Acidic Protein (GFAP)

Tested Reactivity Hu, Ms, Rat, Cat, Dog
Predict Reactivity Chk, Cow, Hrs, Pig
Tested Application ICC/IF, IHC-FoFr , IHC-Fr, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name GFAP
Antigen Species Bovine
Immunogen Recombinant and purified bovine GFAP
Conjugation Un-conjugated
Alternate Names Glial fibrillary acidic protein; ALXDRD; GFAP

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:1,000
IHC-FoFr Assay-dependent
IHC-Fr1:500 - 1:2000
IHC-P1:500 - 1:2000
WB1:5,000
Application Note Specific for the ~50kDa GFAP protein. A lower band at ~45kDa is a proteolytic fragment derived from the GFAP molecule.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Neat Serum
Buffer Neat serum
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14580 Mouse GFAP

GeneID: 24387 Rat GFAP

GeneID: 2670 Human GFAP

Gene Symbol GFAP
Gene Full Name glial fibrillary acidic protein
Background GFAP is one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
Function GFAP is a class-III intermediate filament. It is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30006 NSC and Astrocyte Marker Antibody Duo (GFAP, Nestin)
ARG30007 Astrocyte Marker Antibody Duo (Host: Goat, Rabbit)
Related products:
GFAP antibodies; GFAP Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
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Astrocyte-to-neuron conversion for Parkinson's disease treatment
Research Area Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody
Calculated MW 50 kDa
PTM Phosphorylated by PKN1.

Images (3) Click the Picture to Zoom In

  • ARG52312 anti-GFAP antibody IHC-Fr image

    Immunohistochemistry: Frozen section of Mouse C57BL/6Jnarl brain tissue. The tissue section was fixed by 4% formalin and blocked with BSA with 3% Goat serum, at RT for 1 hour. Tissue section was then stained with ARG52312 anti-GFAP antibody at 1:500 dilution, in PBS with 1% Goat serum, overnight at 4°C.

    Blue: DAPI
    Yellow: Venus reporter gene
    Red: GFAP

  • ARG52312 anti-GFAP antibody ICC/IF image

    Immunofluorescence: Cultured neurons and glia stained with ARG52312 anti-GFAP antibody (red) and ARG52468 anti-Vimentin antibody (green) showing specific labeling of GFAP (red) and vimentin (green). Cells containing GFAP and vimentin appear yellow

  • ARG52312 anti-GFAP antibody WB image

    Western blot: Rat cortex lysate showing specific immunolabeling of the ~50 kDa GFAP protein stained with ARG52312 anti-GFAP antibody.

Customer's Feedback

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Review for anti-GFAP antibody

Application:IHC

Sample:Mouse C57BL/6Jnarl brain tissue

Sample Preparation Method:Frozen

Fixation Buffer:4% formalin

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:Dylight 594

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Review for anti-GFAP antibody

Application:WB

Sample:Mouse brain and Rat brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:5000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Toll-Like Receptor 4 Mediates Methamphetamine-Induced Neuroinflammation through Caspase-11 Signaling Pathway in Astrocytes.

IHC-P, IHC-Fr / Mouse

Du Si-Hao et al.
Front. Mol. Neurosci.,  (2017)

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