ARG10122

anti-GFAP antibody [GF5]

anti-GFAP antibody [GF5] for ELISA,ICC/IF,Immunohistochemistry,Western blot and Human,Mouse,Rat

Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody
nuts_pic
2
anti-GFAP antibody [GF5]

Mouse Monoclonal antibody [GF5] recognizes Glial Fibrillary Acidic Protein (GFAP)

ARG10122 Arigo biolaboratories
5.0 out of 5 based on 2
anti-GFAP antibody [GF5] is rated 5.0 out of 5 by 2.

anti-GFAP antibody [GF5]

Overview

Product Description

Mouse Monoclonal antibody [GF5] recognizes Glial Fibrillary Acidic Protein (GFAP)

Tested Reactivity Hu, Ms, Rat
Tested Application ELISA, ICC/IF, IHC, WB
Specificity This antibody is specific for Human GFAP. There is no cross-reactivity with other neurospecific proteins.
Host Mouse
Clonality Monoclonal
Clone GF5
Isotype IgG2b
Target Name GFAP
Antigen Species Human
Immunogen 43-45 kD band corresponding to GFAP in immunoblotting of extract from human brain and spinal cord.
Conjugation Un-conjugated
Full Name glial fibrillary acidic protein
Alternate Names Glial fibrillary acidic protein; ALXDRD; GFAP

Application Instructions

Application Suggestion
Application Dilution
ELISAAssay-dependent
ICC/IF1:100 - 1:1000
IHC1:100 - 1:1000
WB1:250 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G affinity purified.
Buffer PBS (pH 7.4) and 0.1% Sodium azide
Preservative 0.1% Sodium azide
Concentration 1.0-2.0 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 14580 Mouse GFAP

GeneID: 24387 Rat GFAP

GeneID: 2670 Human GFAP

Gene Symbol GFAP
Background This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
Function GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. [UniProt]
Highlight Related products:
GFAP antibodies; GFAP Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
Microglial help TAM-ing inflammation in the brain
Resrarch Area Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody
PTM Phosphorylated by PKN1.

Images (3) Click the Picture to Zoom In

  • ARG10122 anti-GFAP antibody [GF5] ICC/IF image

    Immunofluorescence: Rat astrocyte primary cell stained with ARG10122 anti-GFAP antibody [GF5] (green) at 1:200 dilution.
    Cell nuclei was stained with DAPI (blue).

  • ARG10122 anti-GFAP antibody [GF5] WB image

    Western blot: 20 µg of Mouse brain and Rat brain lysates stained with ARG10122 anti-GFAP antibody [GF5] at 1:500 dilution.

  • ARG10122 anti-GFAP antibody [GF5] WB image

    Western blot: 30 µg of Rat brain lysate stained with ARG10122 anti-GFAP antibody [GF5] at 1:500 dilution.

Customer's Feedback

nuts_pic      Excellent

Review for anti-GFAP antibody [GF5]

Application:WB

Sample:Mouse brain and Rat brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

show all review

nuts_pic      Excellent

Review for anti-GFAP antibody [GF5]

Application:IF/ICC

Sample:Rat astrocyte primary cell

Fixation Buffer:PFA

Fixation Time:

Fixation Temperature: ºC

Antigen Retrieval Buffer:

Antigen Retrieval Time:

show all review

Clone References

The expression and roles of Nde1 and Ndel1 in the adult mammalian central nervous system.

IHC-Fr / Mouse

Pei Z et al.
Neuroscience.,  (2014)

publication_link

 

hr_line

Impact of age on the cerebrovascular proteomes of wild-type and Tg-SwDI mice.

WB / Mouse

Searcy JL et al.
PLoS One.,  (2014)

publication_link

 

hr_line

Recurrent deletions of ULK4 in schizophrenia: a gene crucial for neuritogenesis and neuronal motility.

Lang B et al.
J Cell Sci.,  (2014)

publication_link

 

hr_line

Differential neuronal expression of receptor interacting protein 3 in rat retina: involvement in ischemic stress response.

IHC-FoFr / Rat

Huang JF et al.
BMC Neurosci.,  (2013)

publication_link

 

hr_line

Involvement of formyl peptide receptors in receptor for advanced glycation end products (RAGE)--and amyloid beta 1-42-induced signal transduction in glial cells.

ICC/IF / Rat

Slowik A et al.
Mol Neurodegener.,  (2012)

publication_link

 

hr_line
Free antibody sample