ARG66801

anti-GFAP antibody [SQab20213]

anti-GFAP antibody [SQab20213] for IHC-Formalin-fixed paraffin-embedded sections and Human

Overview

Product Description Recombinant Rabbit Monoclonal antibody [SQab20213] recognizes GFAP
Tested Reactivity Hu
Tested Application IHC-P
Host Rabbit
Clonality Monoclonal
Clone SQab20213
Isotype IgG
Target Name GFAP
Antigen Species Human
Immunogen Synthetic peptide within aa. 332-432 of Human GFAP.
Conjugation Un-conjugated
Alternate Names Glial fibrillary acidic protein; ALXDRD; GFAP

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:200
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C - 25°C) for 30 minutes.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2670 Human GFAP

Swiss-port # P14136 Human Glial fibrillary acidic protein

Gene Symbol GFAP
Gene Full Name glial fibrillary acidic protein
Background This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
Function GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. [UniProt]
Cellular Localization Cytoplasm. Note=Associated with intermediate filaments. [UniProt]
Calculated MW 50 kDa
PTM Phosphorylated by PKN1. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG66801 anti-GFAP antibody [SQab20213] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human astrocytoma tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66801 anti-GFAP antibody [SQab20213].

  • ARG66801 anti-GFAP antibody [SQab20213] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human brain tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66801 anti-GFAP antibody [SQab20213].