ARG45754
anti-GGA3 antibody
anti-GGA3 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes GGA3 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GGA3 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human GGA3. |
| Conjugation | Un-conjugated |
| Alternate Names | GGA3; Golgi Associated, Gamma Adaptin Ear Containing, ARF Binding Protein 3; KIAA0154; Golgi-Localized, Gamma Ear-Containing, ARF-Binding Protein 3; ADP-Ribosylation Factor-Binding Protein GGA3; Golgi-Associated, Gamma Adaptin Ear Containing, ARF Binding Protein 3 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 90 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9NZ52 Human ADP-ribosylation factor-binding protein GGA3 |
|---|---|
| Gene Symbol | GGA3 |
| Gene Full Name | Golgi Associated, Gamma Adaptin Ear Containing, ARF Binding Protein 3 |
| Background | This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants have been identified in this gene. [provided by RefSeq, Feb 2010] |
| Function | Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif. [UniProt] |
| Cellular Localization | Endosome; Golgi apparatus; Membrane. [UniProt] |
| Calculated MW | 78 kDa |
| PTM | Phosphoprotein; Ubl conjugation. [UniProt] |
Images (4) Click the Picture to Zoom In
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ARG45754 anti-GGA3 antibody IHC-P image
Immunohistochemistry: Human liver cancer stained with ARG45754 anti-GGA3 antibody at 2 μg/ml dilution.
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ARG45754 anti-GGA3 antibody ICC/IF image
Immunofluorescence: CACO-2 stained with ARG45754 anti-GGA3 antibody at 5 μg/ml dilution.
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ARG45754 anti-GGA3 antibody WB image
Western blot: HEL stained with ARG45754 anti-GGA3 antibody at 0.5 μg/ml dilution.
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ARG45754 anti-GGA3 antibody FACS image
Flow Cytometry: Raji stained with ARG45754 anti-GGA3 antibody at 1 µg/10^6 cells dilution.
