ARG10764

anti-GLI2 antibody

anti-GLI2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Overview

Product Description

Rabbit Polyclonal antibody recognizes GLI2

Tested Reactivity Hu, Ms
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name GLI2
Antigen Species Human
Immunogen Synthetic peptide around the N-terminus of Human GLI2. (RNDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCLHVRAIKTE)
Conjugation Un-conjugated
Full Name GLI family zinc finger 2
Alternate Names Tax helper protein; PHS2; HPE9; Zinc finger protein GLI2; THP1; THP2; GLI family zinc finger protein 2; CJS

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P4 - 8 µg/ml
WB1 - 4 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 168 kDa

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.09% (W/V) Sodium azide and 2% Sucrose.
Preservative 0.09% (W/V) Sodium azide
Stabilizer 2% Sucrose
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 14633 Mouse GLI2

GeneID: 2736 Human GLI2

Swiss-port # P10070 Human Zinc finger protein GLI2

Swiss-port # Q0VGT2 Mouse Zinc finger protein GLI2

Gene Symbol GLI2
Gene Full Name GLI family zinc finger 2
Background This gene encodes a protein which belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B. [provided by RefSeq, Jul 2008]
Function Functions as transcription regulator in the hedgehog (Hh) pathway. Functions as transcriptional activator. May also function as transcriptional repressor (By similarity). Requires STK36 for full transcriptional activator activity. Required for normal embryonic development.

Isoform 1, isoform 2, isoform 3 and isoform 4: Act as transcriptional activators in T-cell leukemia virus type 1 (HTLV-1)-infected cells in a Tax-dependent manner. Bind to the DNA sequence 5'-GAACCACCCA-3' which is part of the Tax-responsive element (TRE-2S) regulatory element that augments the Tax-dependent enhancer of HTLV-1. Are involved in the smoothened (SHH) signaling pathway.

Isoform 5: Acts as a transcriptional repressor. [UniProt]
Cellular Localization Nucleus. Cytoplasm. Note=In keratinocytes, it is sequestered in the cytoplasm by SUFU. In the absence of SUFU, it translocates to the nucleus. Isoform 2: Nucleus
PTM Phosphorylated in vitro by ULK3. Phosphorylated by DYRK2; this inhibits GLI2 transcription factor activity and promotes proteasomal degradation of GLI2.
Acetylation at Lys-757 inhibits Hh target gene expression, probably by impeding entry into chromatin thus preventing promoter occupancy.

Images (5) Click the Picture to Zoom In

  • ARG10764 anti-GLI2 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human Intestine (Epithelial cells of intestinal gland) stained with ARG10764 anti-GLI2 antibody at 4 - 8 µg/ml dilution. Magnification: 400X

  • ARG10764 anti-GLI2 antibody WB image

    Western blot: HepG2 cell lysate stained with ARG10764 anti-GLI2 antibody at 1 µg/ml dilution.

  • ARG10764 anti-GLI2 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human heart (Myocardial cells) stained with ARG10764 anti-GLI2 antibody at 4 - 8 µg/ml. Magnification: 400X

  • ARG10764 anti-GLI2 antibody IHC image

    Immunohistochemistry: Mouse gut tissue stained with ARG10764 anti-GLI2 antibody (green) at 1:50 dilution. Red: Neural crest cells.

  • ARG10764 anti-GLI2 antibody WB image

    Western blot: 25 µg of Jurkat cell lysates A) without, or B) with 4 µg/ml blocking peptide. The blots were stained with ARG10764 anti-GLI2 antibody at 2.5 µg/ml dilution.