ARG10781

anti-GLUT1 antibody

anti-GLUT1 antibody for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunohistochemistry (PFA perfusion fixed frozen sections),Immunoprecipitation,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes GLUT1
Tested Reactivity Hu, Ms, Rat
Tested Application ELISA, ICC/IF, IHC-FoFr , IHC-Fr, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name GLUT1
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide around aa. 478-492 of Human GLUT-1. (PEELFHPLGADSQV)
Conjugation Un-conjugated
Full Name solute carrier family 2 (facilitated glucose transporter), member 1
Alternate Names DYT17; HepG2 glucose transporter; CSE; GLUT-1; GLUT; GLUT1DS; DYT18; HTLVR; PED; Glucose transporter type 1, erythrocyte/brain; DYT9; EIG12; GLUT1; Solute carrier family 2, facilitated glucose transporter member 1

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA1:12000
ICC/IF1:50 - 1:200
IHC-FoFr 1:50 - 1:200
IHC-Fr1:50 - 1:200
IHC-P1:50 - 1:200
IP1:200
WB1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 54 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer Tris-Glycine Buffer (pH 7.4 - 7.8), Hepes, 0.02% Sodium azide, 30% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 30% Glycerol and 0.5% BSA
Concentration 0.75 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 20525 Mouse SLC2A1

GeneID: 24778 Rat SLC2A1

GeneID: 6513 Human SLC2A1

Gene Symbol SLC2A1
Gene Full Name solute carrier family 2 (facilitated glucose transporter), member 1
Background This gene encodes a major glucose transporter in the mammalian blood-brain barrier. The encoded protein is found primarily in the cell membrane and on the cell surface, where it can also function as a receptor for human T-cell leukemia virus (HTLV) I and II. Mutations in this gene have been found in a family with paroxysmal exertion-induced dyskinesia. [provided by RefSeq, Apr 2013]
Function Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG10781 anti-GLUT1 antibody WB image

    Western blot: 10 µg of Rat brain cortical extracts on 10% SDS-PAGE. Lane 1, 4, 7, 10) untreated for 21 months, Lane 2, 5, 8, 11) Saline treated for 21 months, and Lane 3, 6, 9, 12) CB treated for 21 months. The blots were stained with ARG10781 anti-GLUT1 antibody at 1:2000 dilution (2 hours at RT).

  • ARG10781 anti-GLUT1 antibody WB image

    Western blot: Recombinant GLUT-1 protein stained with ARG10781 anti-GLUT1 antibody at 1:2000 dilution.