ARG10204

anti-GM-CSF antibody [429] (HRP)

anti-GM-CSF antibody [429] (HRP) for ELISA and Human

Immune System antibody

Overview

Product Description HRP-conjugated Mouse Monoclonal antibody [429] recognizes GM-CSF
Tested Reactivity Hu
Tested Application ELISA
Specificity Does not show any cross reaction with other human cytokines or growth factors tested such as M-CSF, G-CSFR, IL-8, IL-16, IL-1β, TGF-β1 and TNF-α.
Host Mouse
Clonality Monoclonal
Clone 429
Isotype IgG1,k
Target Name GM-CSF
Antigen Species Human
Immunogen Purified recombinant human GM-CSF
Conjugation HRP
Full Name colony stimulating factor 2 (granulocyte-macrophage)
Alternate Names GM-CSF; Granulocyte-macrophage colony-stimulating factor; CSF; GMCSF; Molgramostin; Colony-stimulating factor; Sargramostim

Application Instructions

Application Note ELISA: In combination with a monoclonal capture antibody clone 59 (Cat. No.: ARG10032), this HRP conjugated antibody can be used as a tracer antibody in sandwich ELISA applications for human GM-CSF detection.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G affinity purified
Buffer 0.01M PBS (pH 7.2) and 50% Glycerol
Stabilizer 50% Glycerol
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Keep the antibody in the dark and keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 1437 Human CSF2

Swiss-port # P04141 Human Granulocyte-macrophage colony-stimulating factor

Gene Symbol CSF2
Gene Full Name colony stimulating factor 2 (granulocyte-macrophage)
Background GM-CSF is an extracellular homodimer polyprotein, functioning as a hematopoietic growth factor and immune modulator. It can be produced and act upon a variety of cell types, including T-lymphocytes, B-lymphocytes, monocytes/macrophages, endothelial cells, fibroblasts, stromal cells, mesothelial cells, keratinocytes, osteoblasts, uterine epithelial cells, synoviocytes, mast cells and various solid tumors. GM-CSF stimulates stem cells to produce granulocytes and monocytes to cope with infection. Recombinant GM-CSF has been applied to boost white blood cell in cancer patients after chemotherapy and may also be useful as an immune tonic for anemia and AIDS patients.
Function Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. [UniProt]
Research Area Immune System antibody