ARG10032

anti-GM-CSF antibody [59]

anti-GM-CSF antibody [59] for ELISA and Human

Immune System antibody

Overview

Product Description

Mouse Monoclonal antibody [59] recognizes GM-CSF

Tested Reactivity Hu
Tested Application ELISA
Specificity Does not show any cross reaction with other human cytokines or growth factors tested such as M-CSF, G-CSFR, IL-8, IL-16, IL-1β, TGF-β1 and TNF-α.
Host Mouse
Clonality Monoclonal
Clone 59
Isotype IgG1,k
Target Name GM-CSF
Antigen Species Human
Immunogen Purified recombinant human GM-CSF
Conjugation Un-conjugated
Alternate Names GM-CSF; Granulocyte-macrophage colony-stimulating factor; CSF; GMCSF; Molgramostin; Colony-stimulating factor; Sargramostim

Application Instructions

Application Note ELISA: This antibody can be used as a capture antibody in sandwich ELISA applications for human GM-CSF detection in combination with a monoclonal tracer/detection antibody (HRP-conjugated, Cat. No.: ARG10204). Suggested Capture Coating Dose: 0.3 μg/well; Substrate: TMB.

If the above suggested conditions are followed approximately 2pg/mL of GM-CSF in serum/plasma or medium can be detected.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G affinity purified
Buffer 0.01M PBS (pH 7.2)
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 1437 Human CSF2

Swiss-port # P04141 Human Granulocyte-macrophage colony-stimulating factor

Gene Symbol CSF2
Gene Full Name colony stimulating factor 2 (granulocyte-macrophage)
Background The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]
Function Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. [UniProt]
Resrarch Area Immune System antibody

Clone References

Reduced BMPR2 expression induces GM-CSF translation and macrophage recruitment in humans and mice to exacerbate pulmonary hypertension.

Sawada H et al.
J Exp Med.,  (2014)

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