ARG10091

anti-GP IIb/IIIa antibody [237]

anti-GP IIb/IIIa antibody [237] for Western blot,ELISA and Human

Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [237] recognizes IGP IIb/IIIa
Tested Reactivity Hu
Tested Application ELISA, WB
Host Mouse
Clonality Monoclonal
Clone 237
Isotype IgG1,k
Target Name GP IIb/IIIa
Antigen Species Human
Immunogen Human platelet suspension
Conjugation Un-conjugated
Alternate Names GTA; GT; GPalpha IIb; PPP1R93; CD41; BDPLT2; BDPLT16; GP2B; Integrin alpha-IIb; GPIIb; Platelet membrane glycoprotein IIb; HPA3; CD antigen CD41; CD41B

Application Instructions

Application Note ELISA: The mAb is reactive to platelet coated ELISA plate.

Western Blot: The antibody reacts with GPIIb/IIIa on Western blot with platelet membrane glycoprotein extract.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G affinity purified
Buffer 0.01M PBS (pH 7.2)
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 3674 Human ITGA2B

Swiss-port # P08514 Human Integrin alpha-IIb

Gene Symbol ITGA2B
Gene Full Name integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41)
Background Human platelet membrane glycoprotein Glycoprotein IIb/IIIa (GP IIb/IIIa) is a dominate receptor on the platelet membrane with very high molecular weight (>200kDa). After the platelet is activated, the GP IIb/IIIa receptor complex changes conformation and binds to fibrinogen in blood circulation with high affinity. The activation of platelets is initiated locally when the endothelial layer is wounded and the platelets are exposed to the collagen underneath the endothelium. The fibrinogen aggregates with platelets when binding to the GP IIb/IIIa receptor on platelets, thus primary haemostasis (Platelet clot) is formed.
Function Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface. [UniProt]
Resrarch Area Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody

Clone References

Protein disulfide isomerase directly interacts with β-actin Cys374 and regulates cytoskeleton reorganization.

Sobierajska K et al.
J Biol Chem.,  (2014)

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Effects of protein solution composition on the time-dependent functional activity of fibrinogen on surfaces.

Soman P et al.
Langmuir.,  (2011)

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Substantial changes of cellular iron homeostasis during megakaryocytic differentiation of K562 cells.

WB / Human

Wandzik K et al.
Dev Growth Differ.,  (2009)

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