anti-GPX4 antibody

anti-GPX4 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Ferroptosis/Oxytosis Study antibody
publication_link Publication5


Product Description Rabbit Polyclonal antibody recognizes GPX4
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name GPX4
Antigen Species Human
Immunogen Synthetic peptide derived from Human GPX4.
Conjugation Un-conjugated
Alternate Names Phospholipid hydroperoxide glutathione peroxidase, mitochondrial; EC; MCSP; PHGPx; GSHPx-4; Glutathione peroxidase 4; GPx-4; snPHGPx; snGPx

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 20 kDa


Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 2879 Human GPX4

GeneID: 29328 Rat GPX4

GeneID: 625249 Mouse GPX4

Gene Symbol GPX4
Gene Full Name glutathione peroxidase 4
Background This gene encodes a member of the glutathione peroxidase protein family. Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. The encoded protein has been identified as a moonlighting protein based on its ability to serve dual functions as a peroxidase as well as a structural protein in mature spermatozoa. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Function Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage (By similarity). [UniProt]
Cellular Localization Isoform Mitochondrial: Mitochondrion. Isoform Cytoplasmic: Cytoplasm. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30337 Ferroptosis / Oxytosis Antibody Panel
Related products:
GPX4 antibodies; GPX4 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Ferroptosis/Oxytosis Antibody Panel is launched
Therapeutic strategies against PDAC
Research Area Ferroptosis/Oxytosis Study antibody
Calculated MW 22 kDa (mitochondrial)
20 kDa (cytoplasmic)

Images (2) Click the Picture to Zoom In

  • ARG41400 anti-GPX4 antibody WB image

    Western blot:: Mouse Testis stained with ARG41400 anti-GPX4 antibody at 1:1000 dilution.

    From Lipeng Li et al. Ecotoxicol Environ Saf (2023), doi: 10.1016/j.ecoenv.2023.115309, Fig. 2F1.

  • ARG41400 anti-GPX4 antibody WB image

    Western blot: 20 μg of Mouse testis and Rat testis lysates stained with ARG41400 anti-GPX4 antibody at 1:1000 dilution.

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Sample Loading Amount:20 μg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

A novel quinoline derivative, DFIQ, sensitizes NSCLC cells to ferroptosis by promoting oxidative stress accompanied by autophagic dysfunction and mitochondrial damage

WB / Human

Yung-Ding Bow et al.
Cancer Cell Int.,  (2023)




FDX1 regulates leydig cell ferroptosis mediates PM2.5-induced testicular dysfunction of mice

WB / Mouse

Lipeng Li et al.
Ecotoxicol Environ Saf.,  (2023)




Oyster-Derived Tyr-Ala (YA) Peptide Prevents Lipopolysaccharide/D-Galactosamine-Induced Acute Liver Failure by Suppressing Inflammatory, Apoptotic, Ferroptotic, and Pyroptotic Signals

WB / Mouse

Adrian S Siregar et al.
Mar Drugs.,  (2021)




Blockage of Nrf2 and autophagy by L-selenocystine induces selective death in Nrf2-addicted colorectal cancer cells through p62-Keap-1-Nrf2 axis

WB / Human

Wei-Lun Hsu et al.
Cell Death Dis,  (2022)




Fermented and Aged Ginseng Sprouts ( Panax ginseng) and Their Main Component, Compound K, Alleviate Asthma Parameters in a Mouse Model of Allergic Asthma through Suppression of Inflammation, Apoptosis, ER Stress, and Ferroptosis

WB / Mouse

Ji Hyeon Ryu et al.
Antioxidants (Basel),  (2022)