anti-GSDMD antibody

anti-GSDMD antibody for ICC/IF,Western blot and Human,Mouse,Rat

Pyroptosis Study antibody
publication_link Publication3


Product Description Rabbit Polyclonal antibody recognizes GSDMD
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, WB
Specificity The antibody reacts to full length and N-terminal fragment (1-275) of GSDMD protein.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name GSDMD
Antigen Species Mouse
Immunogen Recombinant fusion protein within aa. 1-275 of GSDMD.
Conjugation Un-conjugated
Alternate Names FKSG10; DF5L; Gasdermin domain-containing protein 1; Gasdermin-D; DFNA5L; GSDMDC1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse lung
Observed Size ~ 53 kDa


Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 69146 Mouse GSDMD

GeneID: 79792 Human GSDMD

Swiss-port # P57764 Human Gasdermin-D

Swiss-port # Q9D8T2 Mouse Gasdermin-D

Gene Symbol GSDMD
Gene Full Name gasdermin D
Background GSDMD (Gasdermin D) is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]
Function GSDMD, N-terminal: Promotes pyroptosis in response to microbial infection and danger signals. Produced by the cleavage of gasdermin-D by inflammatory caspases CASP1 or CASP4 in response to canonical, as well as non-canonical (such as cytosolic LPS) inflammasome activators (PubMed:26375003, PubMed:26375259, PubMed:27418190). After cleavage, moves to the plasma membrane where it strongly binds to inner leaflet lipids, including monophosphorylated phosphatidylinositols, such as phosphatidylinositol 4-phosphate, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate, as well as phosphatidylinositol (3,4,5)-bisphosphate, and more weakly to phosphatidic acid and phosphatidylserine (PubMed:27281216). Homooligomerizes within the membrane and forms pores of 10 - 15 nanometers (nm) of inner diameter, possibly allowing the release of mature IL1B and triggering pyroptosis (PubMed:27418190, PubMed:27281216). Exhibits bactericidal activity. Gasdermin-D, N-terminal released from pyroptotic cells into the extracellular milieu rapidly binds to and kills both Gram-negative and Gram-positive bacteria, without harming neighboring mammalian cells, as it does not disrupt the plasma membrane from the outside due to lipid-binding specificity (PubMed:27281216). Under cell culture conditions, also active against intracellular bacteria, such as Listeria monocytogenes. Strongly binds to bacterial and mitochondrial lipids, including cardiolipin. Does not bind to unphosphorylated phosphatidylinositol, phosphatidylethanolamine nor phosphatidylcholine (PubMed:27281216). [UniProt]
Cellular Localization Gasdermin-D: Cytoplasm, cytosol. Inflammasome. Note=In response to a canonical inflammasome stimulus, such as nigericin, recruited to NLRP3 inflammasone with similar kinetics to that of uncleaved CASP1 precursor. Gasdermin-D, N-terminal: Cell membrane. Secreted. Note=Released in the extracellular milieu following pyroptosis. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30330 Pyroptosis Antibody Panel
Related products:
GSDMD antibodies; GSDMD Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Exploring Antiviral Immune Response
RIP1 activation and pathogenesis of NASH
Solutions for studying PANoptosis & PANoptosome
Research Area Pyroptosis Study antibody
Calculated MW 53 kDa
PTM Cleavage at Asp-275 by CASP1 (mature and uncleaved precursor forms) or CASP4 relieves autoinhibition and is sufficient to initiate pyroptosis. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG41404 anti-GSDMD antibody ICC/IF image

    Immunofluorescence: RAW264.7 cells stained with ARG41404 anti-GSDMD antibody at 1:100 dilution.

  • ARG41404 anti-GSDMD antibody WB image

    Western blot: 25 µg of Mouse lung lysate stained with ARG41404 anti-GSDMD antibody at 1:1000 dilution.

Specific References

Curcumin improves the function of umbilical vein endothelial cells by inhibiting H2O2‑induced pyroptosis

WB / Human

Yulin Yuan et al.
Mol Med Rep,  (2022)




Curcumin Ameliorates White Matter Injury after Ischemic Stroke by Inhibiting Microglia/Macrophage Pyroptosis through NF- κ B Suppression and NLRP3 Inflammasome Inhibition

WB / Mouse

Yuanyuan Ran et al.
Oxid Med Cell Longev.,  (2021)




Aronia Melanocarpa Polysaccharide Ameliotates Inflammation and Aging in Mice by Modulating AMPK/SIRT1/NF-κB Signaling Pathway and Gut Microbiota

WB / Mouse

Yingchun Zhao et al.
Preprint.,  (2021)