ARG51511

anti-GSK3 alpha phospho (Ser21) antibody

anti-GSK3 alpha phospho (Ser21) antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human,Mouse,Rat

Cell Biology and Cellular Response antibody; Cell Death antibody; Developmental Biology antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes GSK3 alpha phospho (Ser21)
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name GSK3 alpha
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of serine 21 (T-S-S(p)-F-A) derived from Human GSK3α.
Conjugation Un-conjugated
Alternate Names Glycogen synthase kinase-3 alpha; EC 2.7.11.26; Serine/threonine-protein kinase GSK3A; GSK-3 alpha; EC 2.7.11.1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100 - 1:200
IHC-P1:50 - 1:100
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2931 Human GSK3A

GeneID: 50686 Rat GSK3A

GeneID: 606496 Mouse GSK3A

Gene Symbol GSK3A
Gene Full Name glycogen synthase kinase 3 alpha
Background Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN.
Function Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1. Requires primed phosphorylation of the majority of its substrates. Contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Regulates glycogen metabolism in liver, but not in muscle. May also mediate the development of insulin resistance by regulating activation of transcription factors. In Wnt signaling, regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin. Facilitates amyloid precursor protein (APP) processing and the generation of APP-derived amyloid plaques found in Alzheimer disease. May be involved in the regulation of replication in pancreatic beta-cells. Is necessary for the establishment of neuronal polarity and axon outgrowth. Through phosphorylation of the anti-apoptotic protein MCL1, may control cell apoptosis in response to growth factors deprivation. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Cell Death antibody; Developmental Biology antibody; Signaling Transduction antibody
Calculated MW 51 kDa
PTM Phosphorylated by AKT1 at Ser-21: upon insulin-mediated signaling, the activated PKB/AKT1 protein kinase phosphorylates and desactivates GSK3A, resulting in the dephosphorylation and activation of GYS1. Activated by phosphorylation at Tyr-279.

Images (4) Click the Picture to Zoom In

  • ARG51511 anti-GSK3 alpha phospho (Ser21) antibody WB image

    Western blot: Extracts from 293 cells untreated(lane 1) or treated with serum(lane 2) stained with ARG51511 anti-GSK3 alpha phospho (Ser21) antibody.

  • ARG51511 anti-GSK3 alpha phospho (Ser21) antibody WB image

    Western blot: Extracts from 293 cells, treated with insulin or calf intestinal phosphatase (CIP), stained with ARG51511 anti-GSK3 alpha phospho (Ser21) antibody.

  • ARG51511 anti-GSK3 alpha phospho (Ser21) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51511 anti-GSK3 alpha phospho (Ser21) antibody (left) or the same antibody preincubated with blocking peptide (right).

  • ARG51511 anti-GSK3 alpha phospho (Ser21) antibody ICC/IF image

    Immunofluorescence: methanol-fixed HeLa cells showing cytoplasmic staining stained with ARG51511 anti-GSK3 alpha phospho (Ser21) antibody.