ARG66338

anti-GST tag antibody [SQab1886]

anti-GST tag antibody [SQab1886] for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Other

Overview

Product Description Recombinant Rabbit Monoclonal antibody [SQab1886] recognizes GST tag
Tested Reactivity Other
Tested Application FACS, ICC/IF, IP, WB
Host Rabbit
Clonality Monoclonal
Clone SQab1886
Isotype IgG
Target Name GST tag
Antigen Species Others
Immunogen Recombinant full length protein within aa. 1-218 of Schistosoma japonicum GST.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:800 - 1:2000
ICC/IF1:10000 - 1:40000
IP1:50
WB1:2000 - 1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (6) Click the Picture to Zoom In

  • ARG66338 anti-GST tag antibody [SQab1886] ICC/IF image

    Immunofluorescence: 293 cells transfected with N-terminal GST tagged gene. Cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66338 anti-GST tag antibody [SQab1886] (red) at 1:40,000 and 4°C. DAPI (blue) was used as the nuclear counter stain.

  • ARG66338 anti-GST tag antibody [SQab1886] FACS image

    Flow Cytometry: 293 cells transfected with C-terminal (red) and N-terminal (blue) GST tagged gene. The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66338 anti-GST tag antibody [SQab1886] at 1:2,000 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control. .

  • ARG66338 anti-GST tag antibody [SQab1886] WB image

    Western blot: 1) 2 µg of 293 cell lysates transfected with N-terminal GST tagged gene, 2) 2 µg of 293 cell lysates transfected with C-terminal GST tagged gene, 3) 10 µg of Mock 293 cell lysates stained with ARG66338 anti-GST tag antibody [SQab1886] at 1:10000 dilution, and 4) 20 ng of two fusion proteins, one (45 kDa) with GST tag on C-terminal, the other (83 kDa) with GST tag on N-terminal stained with ARG66338 anti-GST tag antibody [SQab1886] at 1:2000 dilution.

  • ARG66338 anti-GST tag antibody [SQab1886] IP image

    Immunoprecipitation: 0.2 mg of 293 whole cell lysates transfected with C-terminal GST tagged gene immunoprecipitated (1:50) and stained with ARG66338 anti-GST tag antibody [SQab1886]. 1) ARG66338 IP in 293 whole cell lysates transfected with C-terminal GST tagged gene, 2) PBS instead of ARG66338 in 293 whole cell lysates transfected with C-terminal GST tagged gene, and 3) 293 whole cell lysate transfected with C-terminal GST tagged gene, 2 µg (input).

  • ARG66338 anti-GST tag antibody [SQab1886] ICC/IF image

    Immunofluorescence: 293 cells transfected with C-terminal GST tagged gene. Cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66338 anti-GST tag antibody [SQab1886] (red) at 1:40,000 and 4°C. DAPI (blue) was used as the nuclear counter stain.

  • ARG66338 anti-GST tag antibody [SQab1886] IP image

    Immunoprecipitation: 0.2 mg of 293 whole cell lysates transfected with N-terminal GST tagged gene immunoprecipitated (1:50) and stained with ARG66338 anti-GST tag antibody [SQab1886]. 1) ARG66338 IP in 293 whole cell lysates transfected with N-terminal GST tagged gene, 2) PBS instead of ARG66338 in 293 whole cell lysates transfected with N-terminal GST tagged gene, and 3) 293 whole cell lysates transfected with N-terminal GST tagged gene, 2 µg (input).