ARG10713

anti-Galectin 3 antibody [5C21]

anti-Galectin 3 antibody [5C21] for ICC/IF,IHC-Frozen sections,Western blot and Human,Rat

Overview

Product Description

Mouse Monoclonal antibody [5C21] recognizes Galectin 3

Tested Reactivity Hu, Rat
Predict Reactivity Ms
Tested Application ICC/IF, IHC-Fr, WB
Host Mouse
Clonality Monoclonal
Clone 5C21
Isotype IgG1
Target Name Galectin 3
Immunogen Full length recombinant Galectin-3 expressed in and purified from E. coli.
Conjugation Un-conjugated
Full Name lectin, galactoside-binding, soluble, 3
Alternate Names Laminin-binding protein; Gal-3; L-31; GALBP; Galactoside-binding protein; MAC2; GAL3; GALIG; Mac-2 antigen; CBP 35; Galectin-3; CBP35; Galactose-specific lectin 3; IgE-binding protein; L31; LGALS2; 35 kDa lectin; Carbohydrate-binding protein 35; Lectin L-29

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:2000
IHC-Fr1:2000
WB1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification.
Buffer PBS and 50% Glycerol.
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 3958 Human LGALS3

GeneID: 83781 Rat LGALS3

Swiss-port # P08699 Rat Galectin-3

Swiss-port # P17931 Human Galectin-3

Gene Symbol LGALS3
Gene Full Name lectin, galactoside-binding, soluble, 3
Background This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]
Function Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. [UniProt]

Images (4) Click the Picture to Zoom In

  • ARG10713 anti-Galectin 3 antibody [5C21] ICC/IF image

    Immunocytochemistry: Rat brain neural cultures stained with ARG10713 anti-Galectin 3 antibody [5C21] (green) and DNA (blue). Staining can be seen in several types of glia and lymphocytic cells, including these cells which have the morphology of microglia. Surrounding cells reveal no Galectin-3 staining.

  • ARG10713 anti-Galectin 3 antibody [5C21] ICC/IF image

    Immunofluorescence: Cortical neuron-glial cells from E20 Rat stained with ARG10713 anti-Galectin 3 antibody [5C21] (red) at 1:1000 dilution and costained with ARG52328 anti-MAP2 antibody (green) at 1:10000 dilution. Hoechst (blue) for nuclear staining.

    Clone 5C21 produces strong staining of microglia (top panel), other glial cells (bottom panel) and lymphocytic cells (small round cells), while the MAP2 antibody labels dendrites and perikarya of mature neurons.

  • ARG10713 anti-Galectin 3 antibody [5C21] WB image

    Western blot: crude HeLa cell extract stained with ARG10713 anti-Galectin 3 antibody [5C21].

  • ARG10713 anti-Galectin 3 antibody [5C21] WB image

    Western blot: Mouse brain, Rat brain, Rat liver, Rat heart, Rat kidney, Rat lung and HEK293 cell lysates stained with ARG10713 anti-Galectin 3 antibody [5C21] (green) at 1:2000 dilution. The same blot was simultaneously stained with ARG10757 anti-Hsp 60 antibody (red) at 1:20000 dilution.