ARG45589
anti-Gelsolin antibody
anti-Gelsolin antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Monkey
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Gelsolin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Mk |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Gelsolin |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human Gelsolin. |
| Conjugation | Un-conjugated |
| Alternate Names | GSN; gelsolin; ADF; Actin-depolymerizing factor; Brevin; AGEL; Gelsolin |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 86 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GSN |
| Gene Full Name | gelsolin |
| Background | The protein encoded by this gene binds to the "plus" ends of actin monomers and filaments to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis. [UniProt] |
| Cellular Localization | Isoform 2: Cytoplasm, cytoskeleton.. [UniProt] |
| Calculated MW | 86 kDa |
| PTM | Phosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids.. [UniProt] |
Images (4) Click the Picture to Zoom In
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ARG45589 anti-Gelsolin antibody WB image
Western blot: Hela and THP-1 stained with ARG45589 anti-Gelsolin antibody at 0.5 μg/ml dilution.
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ARG45589 anti-Gelsolin antibody WB image
Western blot: RAW264.7 stained with ARG45589 anti-Gelsolin antibody at 0.5 μg/ml dilution.
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ARG45589 anti-Gelsolin antibody IHC-P image
Immunohistochemistry: Rat spleen stained with ARG45589 anti-Gelsolin antibody at 1 μg/ml dilution.
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ARG45589 anti-Gelsolin antibody WB image
Western blot: COS-7 stained with ARG45589 anti-Gelsolin antibody at 0.5 μg/ml dilution.
