ARG22247

anti-GluR1 antibody [S355-1]

anti-GluR1 antibody [S355-1] for Western blot,Immunohistochemistry,ICC/IF and Human,Mouse,Rat

Neuroscience antibody; Postsynaptic Receptor antibody

Overview

Product Description Mouse Monoclonal antibody [S355-1] recognizes GluR1
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC, WB
Specificity Detects ~100kDa. Does not cross-react with GluR2.
Host Mouse
Clonality Monoclonal
Clone S355-1
Isotype IgG1
Target Name GluR1
Antigen Species Rat
Immunogen Fusion protein around aa. 1-389 (extracellular N-terminus) of Rat GluR1
Conjugation Un-conjugated
Full Name glutamate receptor, ionotropic, AMPA 1
Alternate Names GLUH1; GluA1; GluR-1; Glutamate receptor ionotropic, AMPA 1; GluR-K1; GLUR1; HBGR1; AMPA-selective glutamate receptor 1; GluR-A; GLURA; Glutamate receptor 1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100
IHCAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.1% Sodium azide and 50% Glycerol
Preservative 0.1% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 14799 Mouse GRIA1

GeneID: 2890 Human GRIA1

GeneID: 50592 Rat GRIA1

Gene Symbol Gria1
Gene Full Name glutamate receptor, ionotropic, AMPA 1
Background Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate. [UniProt]
Cellular Localization Cell Junction, Cell membrane, Endoplasmic Reticulum
Highlight Related products:
GluR1 antibodies; GluR1 Duos / Panels; Anti-Mouse IgG secondary antibodies;
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Viral-like capsids, new trans-synaptic mRNA transport mechanism
Research Area Neuroscience antibody; Postsynaptic Receptor antibody
Calculated MW 102 kDa
PTM Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis (By similarity).
Phosphorylated at Ser-645. Phosphorylated at Ser-710 by PKC. Phosphorylated at Ser-849 by PKC, PKA and CAMK2. Phosphorylated at Ser-863 by PKC, PKA and PRKG2.

Images (2) Click the Picture to Zoom In

  • ARG22247 anti-GluR1 antibody [S355-1] ICC/IF image

    Immunofluorescence: Human Neuroblastoma cell line SK-N-BE. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: ARG22247 anti-GluR1 antibody [S355-1] at 1:100 for 60 min at RT. Secondary Antibody: Goat anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ARG22247 anti-GluR1 antibody [S355-1] (D) Composite.

  • ARG22247 anti-GluR1 antibody [S355-1] WB image

    Western blot: 1) MW ladder, 2) 10 µg of Rat brain membrane lysates stained with ARG22247 anti-GluR1 antibody [S355-1] at 1:2000 dilution (60 min at RT). Block: 5% milk + TBST O/N at 4°C.