ARG10605

anti-GluR2 antibody

anti-GluR2 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Mouse,Rat

Overview

Product Description

Rabbit Polyclonal antibody recognizes GluR2

Tested Reactivity Ms, Rat
Predict Reactivity Hu
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Target Name GluR2
Antigen Species Human
Immunogen Recombinant protein around aa. 25-360 of Human GluR2
Conjugation Un-conjugated
Full Name glutamate receptor, ionotropic, AMPA 2
Alternate Names AMPA-selective glutamate receptor 2; GluA2; GluR-K2; Glutamate receptor ionotropic, AMPA 2; GluR-2; HBGR2; GLUR2; GluR-B; Glutamate receptor 2; GLURB

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P0.5 - 1 µg/ml
WB0.5 - 1 µg/ml
Application Note Antigen Retrieval for IHC-P: Boiling the paraffin sections in 10mM Citrate buffer, pH6.0, for 20 min
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 99 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.025% Sodium azide and 2.5% BSA.
Preservative 0.025% Sodium azide
Stabilizer 2.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 14800 Mouse GRIA2

GeneID: 29627 Rat GRIA2

Swiss-port # P19491 Rat Glutamate receptor 2

Swiss-port # P23819 Mouse Glutamate receptor 2

Gene Symbol GRIA2
Gene Full Name glutamate receptor, ionotropic, AMPA 2
Background Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, GRIA1-4. The subunit encoded by this gene (GRIA2) is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to render the channel impermeable to Ca(2+). Human and animal studies suggest that pre-mRNA editing is essential for brain function, and defective GRIA2 RNA editing at the Q/R site may be relevant to amyotrophic lateral sclerosis (ALS) etiology. Alternative splicing, resulting in transcript variants encoding different isoforms, (including the flip and flop isoforms that vary in their signal transduction properties), has been noted for this gene. [provided by RefSeq, Jul 2008]
Function Receptor for glutamate that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate. [UniProt]
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PTM Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis (By similarity).

Images (3) Click the Picture to Zoom In

  • ARG10605 anti-GluR2 antibody IHC-P image

    Immunohistochemistry: Mouse brain tissue stained with ARG10605 anti-GluR2 antibody.

  • ARG10605 anti-GluR2 antibody WB image

    Western blot: 1) Rat brain, and 2) Mouse brain lysate stained with ARG10605 anti-GluR2 antibody.

  • ARG10605 anti-GluR2 antibody IHC-P image

    Immunohistochemistry: Rat brain tissue stained with ARG10605 anti-GluR2 antibody.