ARG55691

anti-Glutamine Synthetase antibody

anti-Glutamine Synthetase antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

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anti-Glutamine Synthetase antibody

Rabbit Polyclonal antibody recognizes Glutamine Synthetase

ARG55691 Arigo biolaboratories
3.5 out of 5 based on 2
anti-Glutamine Synthetase antibody is rated 3.5 out of 5 by 2.

anti-Glutamine Synthetase antibody

Overview

Product Description

Rabbit Polyclonal antibody recognizes Glutamine Synthetase

Tested Reactivity Hu, Ms, Rat
Predict Reactivity Bov, Chk, Mk, Pig
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Glutamine Synthetase
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 70-96 (N-terminus) of Human Glutamine Synthetase.
Conjugation Un-conjugated
Full Name glutamate-ammonia ligase
Alternate Names GS; PIG43; Glutamate decarboxylase; EC 6.3.1.2; PIG59; Glutamine synthetase; Glutamate--ammonia ligase; GLNS; EC 4.1.1.15

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:10 - 1:50
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Jurkat

Properties

Form Liquid
Purification Purification with Protein A and immunogen peptide.
Buffer PBS and 0.09% (W/V) Sodium azide.
Preservative 0.09% (W/V) Sodium azide.
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 14645 Mouse GLUL

GeneID: 24957 Rat GLUL

GeneID: 2752 Human GLUL

Gene Symbol GLUL
Gene Full Name glutamate-ammonia ligase
Background The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
Function This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts. [UniProt]
Cellular Localization Cytoplasm. Mitochondrion.
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Images (2) Click the Picture to Zoom In

  • ARG55691 anti-Glutamine Synthetase antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human liver tissue stained with ARG55691 anti-Glutamine Synthetase antibody at 1:25 dilution.

  • ARG55691 anti-Glutamine Synthetase antibody WB image

    Western blot: 20 µg of Jurkat cell lysate stained with ARG55691 anti-Glutamine Synthetase antibody at 1:1000 dilution.

Customer's Feedback

nuts_pic      Good

Review for anti-Glutamine Synthetase antibody

Application:WB

Sample:Mouse brain and Rat brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Average

Review for anti-Glutamine Synthetase antibody

Application:WB

Sample:HeLa

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC