ARG52321

anti-Glycine Receptor antibody

anti-Glycine Receptor antibody for Immunohistochemistry,Western blot and Mouse,Rat

Neuroscience antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Glycine Receptor
Tested Reactivity Ms, Rat
Tested Application IHC, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Glycine Receptor
Antigen Species Rat
Immunogen Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH
Conjugation Un-conjugated
Alternate Names Glycine receptor 48 kDa subunit; STHE; HKPX1; Glycine receptor strychnine-binding subunit; Glycine receptor subunit alpha-1

Application Instructions

Application Suggestion
Tested Application Dilution
IHCfrozen sections: 1:100
WB1:1,00
Application Note Specific for the ~48k α1- and α2-subunits of the glycine receptor in Western blots of Rat spinal cord and brain stem and in cell extracts. Immunolabeling blocked by preadsorption of antibody with the peptide immunogen. Does not recognize other glycine receptor subunits.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Powder
Purification Affinity Purified
Buffer lyophilized
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14654 Mouse GLRA1

GeneID: 25674 Rat GLRA1

Swiss-port # P07727 Rat Glycine receptor subunit alpha-1

Swiss-port # Q64018 Mouse Glycine receptor subunit alpha-1

Gene Symbol GLRA1
Gene Full Name glycine receptor, alpha 1
Background Glycine is an important inhibitory transmitter in the brainstem and spinal cord. Glycine receptors are members of the ligand-gated ion channel family (LGICs) that mediate rapid chemical neurotransmission (Schofield et al., 2003). The binding of glycine to its receptor produces a large increase in chloride conductance, which causes membrane hyperpolarization. Glycine receptors are anchored at inhibitory chemical synapses by a cytoplasmic protein, gephyrin (Fischer et al., 2000). The glycine receptor has been used to great advantage in the identification of the binding sites for alcohol on the LGIC family of proteins (Beckstead et al., 2001; Mihic et al., 1997). These receptors have also been extremely useful in studies of synaptic clustering of receptors (Craig and Lichtman, 2001). The glycine receptor may also act in concert with an NMDAR subunit to form an excitatory receptor (Chatterton et al., 2002).
Research Area Neuroscience antibody
Calculated MW 53 kDa

Images (1) Click the Picture to Zoom In

  • ARG52321 anti-Glycine Receptor antibody WB image

    Western blot: Rat spinal cord and hippocampal lysates showing specific immunolabeling of the ~48 kDa glycine receptor in spinal cord stained with ARG52321 anti-Glycine Receptor antibody.