anti-Glypican 3 antibody [SQab30329]

anti-Glypican 3 antibody [SQab30329] for IHC-Formalin-fixed paraffin-embedded sections and Human


Product Description Recombinant rabbit Monoclonal antibody [SQab30329] recognizes Glypican 3
Tested Reactivity Hu
Tested Application IHC-P
Host Rabbit
Clonality Monoclonal
Clone SQab30329
Isotype IgG
Target Name Glypican 3
Antigen Species Human
Immunogen Synthetic peptide of Human Glypican 3
Conjugation Un-conjugated
Protein Full Name Glypican-3
Alternate Names GPC3, Glypican 3, OCI 5, SGBS1, SGBS, DGSX, SGB, Intestinal Protein OCI 5, Glypican Proteoglycan 3, Glypican 3, GTR2-2, MXR7, SDYS, Heparan Sulphate Proteoglycan, Secreted Glypican-3, OCI5

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:100
Application Note IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0). Incubate the samples at RT (18-25°C) for 44 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Hepatocellular carcinoma


Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05%BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05%BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Gene Symbol GPC3
Gene Full Name Glypican 3
Background Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]
Function Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression.Inhibits the dipeptidyl peptidase activity of DPP4. [UniProt]
Cellular Localization Cell membrane, Membrane
Calculated MW 66 kDa
PTM This processing is essential for its role in inhibition of hedgehog signaling. A second proteolytic event may result in cleavage of the protein on the cell surface, separating it from the GPI-anchor and leading to its shedding from the cell surface. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG67035 anti-Glypican 3 antibody [SQab30329] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded hepatocellular carcinoma stained with ARG67035 anti-Glypican 3 antibody [SQab30329].