ARG10785

anti-Guanylate Cyclase C antibody

anti-Guanylate Cyclase C antibody for Confocal microscopy (Confocal),Dot blot,ELISA,ICC/IF,Immunohistochemistry,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes Guanylate Cyclase C
Tested Reactivity Hu, Ms, Rat
Tested Application Confocal, Dot, ELISA, ICC/IF, IHC, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Guanylate Cyclase C
Antigen Species Rat
Immunogen Synthetic peptide around the C-terminus from Rat Guanylyl cyclase C.
Conjugation Un-conjugated
Full Name guanylate cyclase 2C
Alternate Names MECIL; STAR; STA receptor; MUCIL; hSTAR; Heat-stable enterotoxin receptor; Intestinal guanylate cyclase; Guanylyl cyclase C; GUC2C; EC 4.6.1.2; DIAR6; GC-C

Application Instructions

Application Suggestion
Tested Application Dilution
Confocal1:200
Dot1:10000
ELISA1:10000
ICC/IF1:200
IHC1:200
WB1:500
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 123 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer Tris-Glycine Buffer (pH 7.4 - 7.8), Hepes, 0.02% Sodium azide, 30% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 30% Glycerol and 0.5% BSA
Concentration 0.65 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 14917 Mouse GUCY2C

GeneID: 2984 Human GUCY2C

Swiss-port # P25092 Human Heat-stable enterotoxin receptor

Swiss-port # Q3UWA6 Mouse Heat-stable enterotoxin receptor

Gene Symbol Gucy2c
Gene Full Name guanylate cyclase 2C
Function Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptides guanylin and uroguanylin. [UniProt]
PTM Glycosylation at Asn-75 and/or Asn-79 is required for interaction with VIP36 while glycosylation at Asn-345 and Asn-402 modulates ligand-mediated GC-C activation.

Images (2) Click the Picture to Zoom In

  • ARG10785 anti-Guanylate Cyclase C antibody IHC image

    Immunohistochemistry: Thin section of Mouse small intestine stained with ARG10785 anti-Guanylate Cyclase C antibody (red). Blue: Nuclei.

  • ARG10785 anti-Guanylate Cyclase C antibody WB image

    Western blot: Recombinant Guanylyl cyclase C protein stained with ARG10785 anti-Guanylate Cyclase C antibody at 1:500 dilution.