anti-HAO1 antibody

anti-HAO1 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat

publication_link Publication1


Product Description Rabbit Polyclonal antibody recognizes HAO1
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name HAO1
Antigen Species Human
Immunogen Recombinant Protein of Human HAO1.
Conjugation Un-conjugated
Alternate Names EC; Glycolate oxidase; HAOX1; GOX; GOX1; Hydroxyacid oxidase 1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse liver


Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 15112 Mouse HAO1

GeneID: 54363 Human HAO1

Swiss-port # Q9UJM8 Human Hydroxyacid oxidase 1

Swiss-port # Q9WU19 Mouse Hydroxyacid oxidase 1

Gene Symbol HAO1
Gene Full Name hydroxyacid oxidase (glycolate oxidase) 1
Background This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site. [provided by RefSeq, Jul 2008]
Function Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate. [UniProt]
Calculated MW 41 kDa

Images (2) Click the Picture to Zoom In

  • ARG57344 anti-HAO1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human liver cancer stained with ARG57344 anti-HAO1 antibody at 1:200 dilution.

  • ARG57344 anti-HAO1 antibody WB image

    Western blot: Mouse liver lysate stained with ARG57344 anti-HAO1 antibody.

Specific References

CRISPR/Cas9-mediated Metabolic Pathway Reprogramming in a Novel Humanized Rat Model Ameliorates Primary Hyperoxaluria Type 1.

WB, IHC-P / Rat

Zheng Rui et al.
Kidney Int.,  (2020)