anti-HBV preS2 antibody [SQab1507]

anti-HBV preS2 antibody [SQab1507] for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and HBV

Cancer antibody; Microbiology and Infectious Disease antibody
publication_link Publication2


Product Description

Mouse Monoclonal antibody [SQab1507] recognizes HBV preS2

Tested Reactivity HBV
Tested Application ELISA, ICC/IF, IHC-P, WB
Specificity This antibody could recognize clinical samples including HBsAg (L-HBs and M-HBs) in serum, plasma, and tissue specimens. The antibody does not react with S-HBs protein.
Host Mouse
Clonality Monoclonal
Clone SQab1507
Target Name HBV preS2
Antigen Species HBV
Immunogen GST-tagged fusion protein around aa. 31-55 of HBV preS2
Conjugation Un-conjugated
Alternate Names large S protein; pre-S1/pre-S2/S; L glycoprotein; L-HBsAG; LHB; large surface protein; major surface antigen

Application Instructions

Application Suggestion
Tested Application Dilution
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.4) and 0.01% Thimerosal.
Preservative 0.01% Thimerosal
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 944569 HBV S

Gene Symbol S
Gene Full Name L-HBsAG
Background Hepatitis B virus (HBV) is a hepadnavirus which has a circular genome composed of partially double-stranded DNA. The HBV surface protein antigens (HBsAg) are comprised of large (LHBs), middle (MHBs) and small (SHBs, also called major) protein. LHBs contains preS1, prS2, and small protein. MHBs does not include preS1 protein and SHBs dose not include preS1 and preS2 proteins. HbsAg and its antibodies have been developed as biomarkers to monitor infection stage. Expression of preS1 and preS2 in tissue or serum are also important to reveal the mechanism of HBV infection.
Function The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assumes fusion between virion membrane and endosomal membrane (Probable). In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein.
The middle envelope protein plays an important role in the budding of the virion. It is involved in the induction of budding in a nucleocapsid independent way. In this process the majority of envelope proteins bud to form subviral lipoprotein particles of 22 nm of diameter that do not contain a nucleocapsid. [UniProt]
Research Area Cancer antibody; Microbiology and Infectious Disease antibody

Images (2) Click the Picture to Zoom In

  • ARG65666 anti-HBV preS2 antibody [SQab1507] WB image

    Western blot: 1) HBV surface protein expressed 293T cell lysate and 2) 293T cell lysate (Mock) stained with ARG65665 anti-HBV preS1 antibody [SQab1506] (left) or ARG65666 anti-HBV preS2 antibody [SQab1507] (right) at 1:2500 dilution.

    Data showed ARG65665 anti-HBV preS1 antibody [SQab1506] reacts with L-HBs protein only and ARG65666 anti-HBV preS2 antibody [SQab1507] reacts with L-HBs and M-HBs proteins.

  • ARG65666 anti-HBV preS2 antibody [SQab1507] ELISA image

    Direct ELISA: Direct ELISA data shows that ARG65666 anti-HBV preS2 antibody [SQab1507] reacts with HBV L-HBs protein but not preS1 fragment, S-HBs protein and BSA.

Specific References

Identification of neutralizing epitopes in the preS2 domain of the hepatitis B virus


KeigoYato et al.
Virus Research,  (2022)




Multiple roles of core protein linker in hepatitis B virus replication.


Liu Kuancheng et al.
PLoS Pathog.,  (2018)