ARG22222

anti-HCN1 antibody [S70-28]

anti-HCN1 antibody [S70-28] for Western blot,Immunohistochemistry,Immunoprecipitation and Human,Mouse,Rat

Overview

Product Description Mouse Monoclonal antibody [S70-28] recognizes HCN1
Tested Reactivity Hu, Ms, Rat
Tested Application IHC, IP, WB
Specificity Detects ~100kDa. No cross-reactivity against HCN2.
Host Mouse
Clonality Monoclonal
Clone S70-28
Isotype IgG1
Target Name HCN1
Antigen Species Rat
Immunogen Fusion protein around aa. 778-910 (C terminus) of Rat HCN1
Conjugation Un-conjugated
Alternate Names EIEE24; Brain cyclic nucleotide-gated channel 1; HAC-2; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1; BCNG-1; BCNG1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC1:1000
IPAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 15165 Mouse HCN1

GeneID: 348980 Human HCN1

GeneID: 84390 Rat HCN1

Gene Symbol Hcn1
Gene Full Name hyperpolarization-activated cyclic nucleotide-gated potassium channel 1
Background The membrane protein encoded by this gene is a hyperpolarization-activated cation channel that contributes to the native pacemaker currents in heart and neurons. The encoded protein can homodimerize or heterodimerize with other pore-forming subunits to form a potassium channel. This channel may act as a receptor for sour tastes. [provided by RefSeq, Oct 2011]
Function Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). May mediate responses to sour stimuli. [UniProt]
Cellular Localization Membrane
Calculated MW 99 kDa

Images (4) Click the Picture to Zoom In

  • ARG22222 anti-HCN1 antibody [S70-28] IHC-Fr image

    Immunohistochemistry: 10% Formalin (12-24 hours at RT) fixed Mouse frozen brain section stained with ARG22222 anti-HCN1 Antibody [S70-28] (brown) at 1:1000 dilution (1 hour). Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500uL for 5 minutes at RT.

  • ARG22222 anti-HCN1 antibody [S70-28] WB image

    Western blot: Rat brain membrane lysate stained with ARG22222 anti-HCN1 Antibody [S70-28] at 1:1000 dilution.

  • ARG22222 anti-HCN1 antibody [S70-28] IHC-Fr image

    Immunohistochemistry: 10% Formalin (12-24 hours at RT) fixed Mouse frozen brain section stained with ARG22222 anti-HCN1 Antibody [S70-28] (brown) at 1:1000 dilution (1 hour). Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500uL for 5 minutes at RT.

  • ARG22222 anti-HCN1 antibody [S70-28] IHC image

    Immunohistochemistry: 10% Formalin (12-24 hours at RT) fixed Mouse Cerebellum stained with ARG22222 anti-HCN1 Antibody [S70-28] (brown) at 1:1000 dilution (1 hour). Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500uL for 5 minutes at RT.