ARG10070

anti-HCV NS3 antibody [113]

anti-HCV NS3 antibody [113] for ELISA,Western blot and HCV

Microbiology and Infectious Disease antibody

Overview

Product Description Mouse Monoclonal antibody [113] recognizes HCV NS3
Tested Reactivity HCV
Tested Application ELISA, WB
Specificity No cross reaction was found with synthetic recombinant core protein C + envelope protein M (residues 1-142 on HCV polyprotein), synthetic core protein C (residues 1-61), synthetic NS-3 protein (residues 1378-1458) and synthetic NS-4a protein (residues 1689-1735)
Host Mouse
Clonality Monoclonal
Clone 113
Isotype IgG1,k
Target Name HCV NS3
Antigen Species HCV
Immunogen A highly antigenic polypeptide consisting of essential sequences of at least 60 residues in length, which were selected from genes encoding the NS-3 region of a Chinese HCV strains
Conjugation Un-conjugated

Application Instructions

Application Note Western Blot: mAb clone 113, when used at concentration of 0.1-1μg/mL, will allow visualization of 100 ng/lane of both recombinant chimeric HCV polyprotein and recombinant NS-3 protein. The mAb works on blots transferred from both reducing and non-reducing PAGE gel.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G affinity purified
Buffer 0.01M PBS (pH 7.2)
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Background Hepatitis C virus (HCV) causes chronic hepatitis and liver cirrhosis in human through blood and body fluid transmission. HCV has a positive sense single RNA genome enclosed in the nucleocapsid made of core protein (capsid protein). The nucleocapsid is covered by an envelope made of lipoproteins (E1 and E2). The 9.6 kb HCV genome has a single open-reading frame, which is to be translated into a single polyprotein. HCV viral proteins are produced after processing the polyprotein. Genes for core protein and envelop proteins are located adjacently at the 5’-end of HCV genome, followed by genes for non-structural proteins including NS2, NS3, NS4A, NS4B, NS5, NS5A and NS5B.
Research Area Microbiology and Infectious Disease antibody