anti-HCV NS4 antibody [497]

anti-HCV NS4 antibody [497] for ELISA,Western blot and HCV

Microbiology and Infectious Disease antibody


Product Description Mouse Monoclonal antibody [497] recognizes Hepatitis C virus NS4
Tested Reactivity HCV
Tested Application ELISA, WB
Specificity No cross reaction with synthetic recombinant core protein C + envelope protein M (residues 1 -142), synthetic core protein C (residues 1 -61), recombinant NS-3 protein (residues 1252 -1477) and synthetic NS-3 protein (residues 1378 -1458).
Host Mouse
Clonality Monoclonal
Clone 497
Isotype IgM, kappa
Target Name HCV NS4
Antigen Species HCV
Immunogen Purified recombinant chimeric HCV polyprotein (555 amino acid residues)
Conjugation Un-conjugated

Application Instructions

Application Note Western Blot: This mAb when used at concentration of 0.5μg/mL, will allow visualization of 0.5μg /lane of synthetic NS-4 peptide and 0.1μg /lane recombinant chimeric HCV polyprotein.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Protein G affinity purified
Buffer 0.01M PBS (pH 7.0)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Background Hepatitis C virus (HCV) causes chronic hepatitis and liver cirrhosis in human through blood and body fluid transmission. HCV has a positive sense single RNA genome enclosed in the nucleocapsid made of core protein (capsid protein). The nucleocapsid is covered by an envelope made of lipoproteins (E1 and E2). The 9.6 kb HCV genome has a single open-reading frame, which is to be translated into a single polyprotein. HCV viral proteins are produced after processing the polyprotein. Genes for core protein and envelop proteins are located adjacently at the 5’-end of HCV genome, followed by genes for non-structural proteins including NS2, NS3, NS4A, NS4B, NS5, NS5A and NS5B.
Research Area Microbiology and Infectious Disease antibody