ARG20180

anti-HDAC1 antibody

anti-HDAC1 antibody for Immunohistochemistry,Immunoprecipitation,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes HDAC1
Tested Reactivity Hu, Ms, Rat
Tested Application IHC, IP, WB
Specificity The antibody detects the 62 kDa histone deacetylase 1. It does not cross-react with other HDAC proteins including HDAC2, 3, 4, 5, 6, 7, and 8.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name HDAC1
Antigen Species Human
Immunogen Synthetic peptide corresponding to the C-terminus of human HDAC1
Conjugation Un-conjugated
Full Name histone deacetylase 1
Alternate Names EC 3.5.1.98; HD1; RPD3L1; Histone deacetylase 1; GON-10; RPD3

Application Instructions

Application Suggestion
Tested Application Dilution
IHC10-20 µg/ml
IP10-20 µg/ml
WB0.5-4 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 55 kDa

Properties

Form Liquid
Purification Affinity Purified Antibody
Buffer PBS (pH 7.2), 30% Glycerol, 0.5% BSA and 0.01% Thimerosal
Preservative 0.01% Thimerosal
Stabilizer 30% Glycerol, 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 297893 Rat HDAC1

GeneID: 3065 Human HDAC1

GeneID: 433759 Mouse HDAC1

Gene Symbol HDAC1
Gene Full Name histone deacetylase 1
Background HDAC1 is an active component of transcriptional corepressor complexes which can be recruited to specific promoter regions via transcriptional factors. HDAC1 catalyzes removal of acetyl-groups from acetyl-lysines of histones and promotes compaction of chromatin in these regions, which leads to the inhibition of gene transcription.
Function Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. Deacetylates NR1D2 and abrogates the effect of KAT5-mediated relieving of NR1D2 transcription repression activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Involved in CIART-mediated transcriptional repression of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex or CRY1 through histone deacetylation. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG20180 anti-HDAC1 antibody WB image

    Western Blot: 1. Mouse small intestine tissue lysate 2. Jurkat cell lysate 3. Rat kidney tissue lysate stained with anti-HDAC1 antibody (ARG20180).

    The antibody detects the 62 kDa histone deacetylase 1. It does not cross-react with other HDAC proteins includi ng HDAC2, 3, 4, 5, 6, 7, and 8.