ARG20182

anti-HDAC5 antibody

anti-HDAC5 antibody for Immunohistochemistry,Immunoprecipitation,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes HDAC5
Tested Reactivity Hu, Ms, Rat
Tested Application IHC, IP, WB
Specificity The antibody detects ~124 kDa HDAC5 with human and mouse samples, and in a lesser extent with rat samples. It does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 6, 7, 8, 9,10, and 11.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name HDAC5
Species Human
Immunogen Synthetic peptide surrounding amino acid 575 of human HDAC-5
Conjugation Un-conjugated
Full Name histone deacetylase 5
Alternate Names HD5; EC 3.5.1.98; Histone deacetylase 5; Antigen NY-CO-9; NY-CO-9

Application Instructions

Application Suggestion
Tested Application Dilution
IHC20 µg/ml
IP20 µg/ml
WB0.5-4 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 122 kDa

Properties

Form Liquid
Purification Affinity Purified Antibody
Buffer PBS (pH 7.2), 30% Glycerol, 0.5% BSA and 0.01% Thimerosal
Preservative 0.01% Thimerosal
Stabilizer 30% Glycerol, 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 10014 Human HDAC5

Swiss-port # Q9UQL6 Human Histone deacetylase 5

Gene Symbol HDAC5
Gene Full Name histone deacetylase 5
Background Human HDAC5 is composed of 1122 amino acid residues. The deacetylase domain of HDAC5 is located at the C-terminal half of the molecule. The N-terminal non-deacetylase domain does not show any significant homology with any published sequence. Both domains are required for HDAC5-mediated repression of gene transcription. HDAC5 interacts with a growing number of transcriptional factors including MEF2A as well as other HDAC proteins. The interacting complexes bind to specific regions of chromatin and regulate gene transcription in these regions.

Images (1) Click the Picture to Zoom In

  • ARG20182 anti-HDAC5 antibody WB image

    Western Blot: Jurkat cell lysate stained with anti-HDAC5 antibody (ARG20182).

    The antibody detects ~124 kDa HDAC5 with human and mouse samples, and in a lesser extent with rat samples. It does not cross-react with other HDAC proteins includi ng HDAC1, 2, 3, 4, 6, 7, 8, 9,10, and 11.