ARG20183

anti-HDAC6 antibody

anti-HDAC6 antibody for Immunohistochemistry,Western blot and Human,Mouse,Rat

Overview

Product Description

Rabbit Polyclonal antibody recognizes HDAC6

Tested Reactivity Hu, Ms, Rat
Tested Application IHC, WB
Specificity The antibody detects ~134 kDa HDAC-6. The antibody does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 5, 7, and 8.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name HDAC6
Antigen Species Mouse
Immunogen Synthetic peptide mapping to the N-terminus of mouse HDAC-6
Conjugation Un-conjugated
Full Name histone deacetylase 6
Alternate Names HD6; EC 3.5.1.98; PPP1R90; CPBHM; JM21; Histone deacetylase 6

Application Instructions

Application Suggestion
Tested Application Dilution
IHC20 µg/ml T
WB0.5-4 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control |Jurkat cell lysate can be used as a positive control.
Calculated MW 131 kDa

Properties

Form Liquid
Purification Affinity Purified Antibody
Buffer PBS (pH 7.2), 30% Glycerol, 0.5% BSA and 0.01% Thimerosal
Preservative 0.01% Thimerosal
Stabilizer 30% Glycerol, 0.5% BSA
Concentration 0.2 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 10013 Human HDAC6

GeneID: 15185 Mouse HDAC6

Swiss-port # Q9UBN7 Human Histone deacetylase 6

Swiss-port # Q9Z2V5 Mouse Histone deacetylase 6

Gene Symbol HDAC6
Gene Full Name histone deacetylase 6
Background Human HDAC6 (1215 a.a. residues) possesses two separate putative catalytic domains, both of which are fully functional and contribute independently to the overall activity of HDAC6. A very potent NES is present at the amino-terminus of HDAC6, which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. HDAC6 may participate in coordinating expression of a group of genes involved in the remoldeling of chromatin during cell differentiation.

Images (1) Click the Picture to Zoom In

  • ARG20183 anti-HDAC6 antibody WB image

    Western Blot: Jurkat cell lysate stained with anti-HDAC6 antibody (ARG20183).

    The antibody detects ~134 kDa HDAC-6. Jurkat cell lysate can be used as a positive control. The antibody does not cross-react with other HDAC proteins includi ng HDAC1, 2, 3, 4, 5, 7, and 8.