ARG51629

anti-HDAC8 phospho (Ser39) antibody

anti-HDAC8 phospho (Ser39) antibody for IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human,Mouse,Rat

Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes HDAC8 phospho (Ser39)
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name HDAC8
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of serine 39 (R-A-S(p)-M-V) derived from Human HDAC8.
Conjugation Un-conjugated
Alternate Names MRXS6; WTS; CDLS5; Histone deacetylase 8; HD8; EC 3.5.1.98; CDA07; RPD3; HDACL1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100 - 1:200
IHC-P1:50 - 1:100
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 363481 Rat HDAC8

GeneID: 55869 Human HDAC8

GeneID: 70315 Mouse HDAC8

Gene Symbol HDAC8
Gene Full Name histone deacetylase 8
Background Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by HDAC8 gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene.
Function Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin. May play a role in smooth muscle cell contractility. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody
Calculated MW 42 kDa
PTM Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4.

Images (2) Click the Picture to Zoom In

  • ARG51629 anti-HDAC8 phospho (Ser39) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human lung carcinoma tissue stained with ARG51629 anti-HDAC8 phospho (Ser39) antibody (left) or the same antibody preincubated with blocking peptide (right).

  • ARG51629 anti-HDAC8 phospho (Ser39) antibody ICC/IF image

    Immunofluorescence: methanol-fixed HeLa cells stained with ARG51629 anti-HDAC8 phospho (Ser39) antibody.