ARG54720

anti-HGF antibody

anti-HGF antibody for Western blot,Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections and Human

Cancer antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes HGF
Tested Reactivity Hu
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Target Name HGF
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 521-554 (C-terminus) of Human HGF (NP_000592.3).
Conjugation Un-conjugated
Alternate Names HPTA; Scatter factor; F-TCF; Hepatocyte growth factor; DFNB39; Hepatopoietin-A; HGFB; SF

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:50
IHC-PAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Ramos

Properties

Purification Protein G purified
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3082 Human HGF

Swiss-port # P14210 Human Hepatocyte growth factor

Gene Symbol HGF
Gene Full Name hepatocyte growth factor (hepapoietin A; scatter factor)
Background Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Function Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. [From Uniprot]
Research Area Cancer antibody; Signaling Transduction antibody
Calculated MW 83 kDa

Images (3) Click the Picture to Zoom In

  • ARG54720 anti-HGF antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human hepatocarcinoma tissue stained with ARG54720 anti-HGF antibody.

  • ARG54720 anti-HGF antibody WB image

    Western blot: Ramos cell lysate stained with ARG54720 anti-HGF antibody.

  • ARG54720 anti-HGF antibody FACS image

    Flow Cytometry: CEM cells stained with ARG54720 anti-HGF antibody (right histogram) or without primary antibody control (left histogram).