ARG53935

anti-HLA A2 antibody [BB7.2] (PE)

anti-HLA A2 antibody [BB7.2] (PE) for Flow cytometry and Human

Immune System antibody

Overview

Product Description PE-conjugated Mouse Monoclonal antibody [BB7.2] recognizes HLA A2
Tested Reactivity Hu
Tested Application FACS
Specificity The clone BB7.2 recognizes an epitope at the C-terminus of alpha-2 helix and a turn on one of the underlying beta strands within the human HLA-A2 histocompatibility antigen.
Host Mouse
Clonality Monoclonal
Clone BB7.2
Isotype IgG2b
Target Name HLA A2
Immunogen HLA-A2 solubilised by papain_x000D_
Conjugation PE
Alternate Names MHC class I antigen A*1; HLAA; HLA class I histocompatibility antigen, A-1 alpha chain

Application Instructions

Application Suggestion
Tested Application Dilution
FACS20 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3105 Human HLA-A

Swiss-port # P30443 Human HLA class I histocompatibility antigen, A-1 alpha chain

Gene Symbol HLA-A
Gene Full Name major histocompatibility complex, class I, A
Background HLA-A2 (44 kDa) is the most frequent HLA-A allele in human ethnic populations. HLA-A, together with HLA-B and HLA-C, represent human HLA class I major histocompatibility (MHC) antigens. These intrinsic membrane glycoproteins are expressed on nucleated cells and noncovalently associate with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence._x000D_
Function Involved in the presentation of foreign antigens to the immune system. [UniProt]
Research Area Immune System antibody
Calculated MW 40 kDa
PTM Polyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system (By similarity).

Images (1) Click the Picture to Zoom In

  • ARG53935 anti-HLA A2 antibody [BB7.2] (PE) FACS image

    Flow Cytometry: Human peripheral blood cells stained with ARG53935 anti-HLA A2 antibody [BB7.2] (PE).

Clone References

Recurrence of melanoma following T cell treatment: continued antigen expression in a tumor that evades T cell recruitment.

Straetemans T et al.
Mol Ther.,  (2015)

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Synergistic inhibition of natural killer cells by the nonsignaling molecule CD94.

Cheent KS et al.
Proc Natl Acad Sci U S A.,  (2013)

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Down-modulation of CD8αβ is a fundamental activity of primate lentiviral Nef proteins.

FACS / Human

Heigele A et al.
J Virol.,  (2012)

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HLA-A2-matched peripheral blood mononuclear cells from type 1 diabetic patients, but not nondiabetic donors, transfer insulitis to NOD-scid/γc(null)/HLA-A2 transgenic mice concurrent with the expansion of islet-specific CD8+ T cells.

FACS / 

Whitfield-Larry F et al.
Diabetes.,  (2011)

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Localization and characterization of serologic epitopes on HLA-A2.

Hogan KT et al.
Hum Immunol.,  (1992)

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