anti-HLA E antibody [MEM-E/07] (Biotin)

anti-HLA E antibody [MEM-E/07] (Biotin) for Flow cytometry and Human

Immune System antibody


Product Description Biotin-conjugated Mouse Monoclonal antibody [MEM-E/07] recognizes HLA E
Tested Reactivity Hu
Tested Application FACS
Specificity The clone MEM-E/07 recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells.
The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules (MHC Class Ia): HLA-B7 (strongly), HLA-B8 (moderately), HLA-B27, -B44 (weakly).
Host Mouse
Clonality Monoclonal
Clone MEM-E/07
Isotype IgG1
Target Name HLA E
Immunogen Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.
Conjugation Biotin
Alternate Names MHC class I antigen E; QA1; EA2.1; HLA-6.2; EA1.2; MHC; HLA class I histocompatibility antigen, alpha chain E

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 12 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Note The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 3133 Human HLA-E

Swiss-port # P13747 Human HLA class I histocompatibility antigen, alpha chain E

Gene Symbol HLA-E
Gene Full Name major histocompatibility complex, class I, E
Background HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. [provided by RefSeq, Jul 2008]
Function Preferably binds to a peptide derived from the signal sequence of most HLA-A, -B, -C and -G molecules. [UniProt]
Research Area Immune System antibody
Calculated MW 40 kDa

Images (1) Click the Picture to Zoom In

  • ARG53945 anti-HLA E antibody [MEM-E/07] (Biotin) FACS image

    Flow Cytometry: HLA-E transfectants stained with ARG53945 anti-HLA E antibody [MEM-E/07] (Biotin) at 4 µg/ml dilution, followed by Streptavidin-APC (red). Blank sample (grey).

Clone References

HLA-E monoclonal antibodies recognize shared peptide sequences on classical HLA class Ia: relevance to human natural HLA antibodies.

ELISA / Human

Ravindranath MH et al.
Mol Immunol.,  (2010)