anti-HLA G antibody [G233]

anti-HLA G antibody [G233] for ELISA,Flow cytometry,Immunoprecipitation and Human

Immune System antibody


Product Description Mouse Monoclonal antibody [G233] recognizes HLA G
Tested Reactivity Hu
Tested Application ELISA, FACS, IP
Specificity The clone G233 recognizes several isoforms of HLA-G expressed in all populations of extravillous trophoblast (cell columns, interstitial trophoblast, endovascular trophoblast, placental bed giant cells). HLA-G belongs to the nonclassical MHC Class I molecules (MHC Class Ib).
G233 has been found not to cross-react with any other MHC Class I antigens (HLA-A, -B, -C, -E, -F).
Host Mouse
Clonality Monoclonal
Clone G233
Isotype IgG2a
Target Name HLA G
Antigen Species Human
Immunogen HLA-A2.1/human beta2-microglobulin double transgenic mice were immunized with murine L cells transfected with both human beta2-microglobulin and HLA-G.
Conjugation Un-conjugated
Alternate Names HLA G antigen; MHC class I antigen G; HLA class I histocompatibility antigen, alpha chain G; MHC-G

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 4 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 3135 Human HLA-G

Swiss-port # P17693 Human HLA class I histocompatibility antigen, alpha chain G

Gene Symbol HLA-G
Gene Full Name major histocompatibility complex, class I, G
Background HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]
Function Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T-lymphocytes, macrophages and mononuclear cells. [UniProt]
Research Area Immune System antibody
Calculated MW 38 kDa

Images (2) Click the Picture to Zoom In

  • ARG10111 anti-HLA G antibody [G233] IP image

    Immunoprecipitation: HLA-G from HLA-G1 transfectants (LCL-HLA-G1) immunoprecipitated by ARG10106 anti-HLA G antibody [MEM-G/9] and protein G. HLA-G was stained with ARG10111 anti-HLA G antibody [G233] in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).

  • ARG10111 anti-HLA G antibody [G233] FACS image

    Flow Cytometry: HLA-G transfectants stained with ARG10111 anti-HLA G antibody [G233], followed by APC-conjugated Goat anti-Mouse antibody.

Clone References

The C-terminal amino acid of the MHC-I heavy chain is critical for binding to Derlin-1 in human cytomegalovirus US11-induced MHC-I degradation.

Cho S et al.
PLoS One.,  (2013)




Identification of circulating nonclassic human leukocyte antigen G (HLA-G)-like molecules in exudates.

Gonzalez A et al.
Clin Chem.,  (2011)




Retrieval of trophoblast cells from the cervical canal for prediction of abnormal pregnancy: a pilot study.

Imudia AN et al.
Hum Reprod.,  (2009)




Melanomas and melanoma cell lines do not express HLA-G, and the expression cannot be induced by gammaIFN treatment.

Frumento G et al.
Tissue Antigens.,  (2000)