ARG10106

anti-HLA G antibody [MEM-G/9]

anti-HLA G antibody [MEM-G/9] for Flow cytometry,Immunoprecipitation,IHC-Frozen sections,ICC/IF,ELISA and Human

Immune System antibody

Overview

Product Description Mouse Monoclonal antibody [MEM-G/9] recognizes HLA G
Tested Reactivity Hu
Species Does Not React With Ms
Tested Application ELISA, FACS, ICC/IF, IHC-Fr, IP
Specificity The clone MEM-G/9 reacts with native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. Reactivity with HLA-G3 was also reported.
MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003).
Host Mouse
Clonality Monoclonal
Clone MEM-G/9
Isotype IgG1
Target Name HLA G
Antigen Species Human
Immunogen Recombinant human HLA-G refolded with beta2-microglobulin and peptide.
Conjugation Un-conjugated
Full Name major histocompatibility complex, class I, G
Alternate Names HLA G antigen; MHC class I antigen G; HLA class I histocompatibility antigen, alpha chain G; MHC-G

Application Instructions

Application Note
Flow Cytometry: Recommended dilution: 1-5 μg/ml Positive control: JEG-3 human choriocarcinoma cell line
Immunoprecipitation:
Immunohistochemistry (frozen sections):
Immunocytochemistry: Recommended dilution: Alexa Fluor® 488 Fab-fragment: 5 μg/ml
ELISA: Application note: The antibody MEM-G/9 has been tested as the capture antibody in a sandwich ELISA for analysis of human HLA-G in combination with antibody B2M-01 or with antibody W6/32. Coating antibody (10 μg/ml) Detection antibody (biotin or peroxidase conjugate; 1 μg/ml)
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified by protein A
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 3135 Human HLA-G

Swiss-port # P17693 Human HLA class I histocompatibility antigen, alpha chain G

Gene Symbol HLA-G
Gene Full Name major histocompatibility complex, class I, G
Background HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]
Function Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T-lymphocytes, macrophages and mononuclear cells. [UniProt]
Research Area Immune System antibody

Images (1) Click the Picture to Zoom In

  • ARG10106 anti-HLA G antibody [MEM-G/9] ICC/IF image

    Immunofluorescence: HLA-G1 transfectants stained with ARG10106 anti-HLA G antibody [MEM-G/9] (green).