anti-HLA G antibody [MEM-G/9]
anti-HLA G antibody [MEM-G/9] for ELISA,Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation and Human
Immune System antibody
|Product Description||Mouse Monoclonal antibody [MEM-G/9] recognizes HLA G|
|Species Does Not React With||Ms|
|Tested Application||ELISA, FACS, ICC/IF, IHC-Fr, IP|
|Specificity||The clone MEM-G/9 reacts with native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. Reactivity with HLA-G3 was also reported.
MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003).
|Target Name||HLA G|
|Immunogen||Recombinant human HLA-G refolded with beta2-microglobulin and peptide.|
|Full Name||major histocompatibility complex, class I, G|
|Alternate Names||HLA G antigen; MHC class I antigen G; HLA class I histocompatibility antigen, alpha chain G; MHC-G|
Flow Cytometry: Recommended dilution: 1-5 μg/ml Positive control: JEG-3 human choriocarcinoma cell line
Immunohistochemistry (frozen sections):
Immunocytochemistry: Recommended dilution: Alexa Fluor® 488 Fab-fragment: 5 μg/ml
ELISA: Application note: The antibody MEM-G/9 has been tested as the capture antibody in a sandwich ELISA for analysis of human HLA-G in combination with antibody B2M-01 or with antibody W6/32. Coating antibody (10 μg/ml) Detection antibody (biotin or peroxidase conjugate; 1 μg/ml)
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Purification||Purified by protein A|
|Purity||> 95% (by SDS-PAGE)|
|Buffer||PBS (pH 7.4) and 15 mM Sodium azide|
|Preservative||15 mM Sodium azide|
|Storage instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||major histocompatibility complex, class I, G|
|Background||HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]|
|Function||Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T-lymphocytes, macrophages and mononuclear cells. [UniProt]|
|Research Area||Immune System antibody|
Images (2) Click the Picture to Zoom In
ARG10106 anti-HLA G antibody [MEM-G/9] ICC/IF image
Immunofluorescence: HLA-G1 transfectants stained with ARG10106 anti-HLA G antibody [MEM-G/9] (green).
ARG10106 anti-HLA G antibody [MEM-G/9] IP image
Immunoprecipitation: HLA-G from HLA-G1 transfectants (LCL-HLA-G1) immunoprecipitated by ARG10106 anti-HLA G antibody [MEM-G/9] and protein G. HLA-G was stained with ARG10111 anti-HLA G antibody [G233] in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).