anti-HLA G antibody [MEM-G/9] (Azide free)

anti-HLA G antibody [MEM-G/9] (Azide free) for ELISA,Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation and Human

Immune System antibody


Product Description Azide free Mouse Monoclonal antibody [MEM-G/9] recognizes HLA G
Tested Reactivity Hu
Species Does Not React With Ms
Tested Application ELISA, FACS, ICC/IF, IHC-Fr, IP
Specificity The clone MEM-G/9 reacts with native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. Reactivity with HLA-G3 was also reported.
MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003).
Host Mouse
Clonality Monoclonal
Clone MEM-G/9
Isotype IgG1
Target Name HLA G
Antigen Species Human
Immunogen Recombinant human HLA-G refolded with beta2-microglobulin and peptide.
Conjugation Un-conjugated
Alternate Names HLA G antigen; MHC class I antigen G; HLA class I histocompatibility antigen, alpha chain G; MHC-G

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA10 µg/ml
FACS1 - 5 µg/ml
ICC/IF2 - 5 µg/ml
IHC-Fr5 - 10 µg/ml
Application Note ELISA: Application note: The clone MEM-G/9 has been tested as the capture antibody in a sandwich ELISA for analysis of human HLA-G in combination with clone B2M-01 or with clone W6/32. Coating antibody (10 μg/ml); Detection antibody (biotin or peroxidase conjugate; 1 μg/ml)
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control FACS: JEG-3 Human choriocarcinoma cell line


Form Liquid
Purification Purification with Protein A.
Purification Note 0.2 µm filter sterilized.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 3135 Human HLA-G

Swiss-port # P17693 Human HLA class I histocompatibility antigen, alpha chain G

Gene Symbol HLA-G
Gene Full Name major histocompatibility complex, class I, G
Background HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]
Function Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T-lymphocytes, macrophages and mononuclear cells. [UniProt]
Research Area Immune System antibody
Calculated MW 38 kDa

Images (1) Click the Picture to Zoom In

  • ARG10093 anti-HLA G antibody [MEM-G/9] (Azide free) ICC/IF image

    Immunofluorescence: HLA-G1 transfectants stained with ARG10093 anti-HLA G antibody [MEM-G/9] (Azide free) (green).