anti-HLA G antibody [SQab30313]

anti-HLA G antibody [SQab30313] for IHC-Formalin-fixed paraffin-embedded sections and Human


Product Description Recombinant rabbit Monoclonal antibody [SQab30313] recognizes HLA G
Tested Reactivity Hu
Tested Application IHC-P
Host Rabbit
Clonality Monoclonal
Clone SQab30313
Isotype IgG
Target Name HLA G
Antigen Species Human
Immunogen Synthetic peptide of Human HLA G.
Conjugation Un-conjugated
Protein Full Name HLA class I histocompatibility antigen, alpha chain G
Alternate Names HLA-G, Major Histocompatibility Complex, Class I, G, HLA Class I Histocompatibility Antigen, Alpha Chain G, HLA-G Histocompatibility Antigen, Class I, G, MHC Class I Antigen G, B2 Microglobulin, HLA G Antigen, Mutant MHC Class Ib Antigen, Mutant MHC Class I Antigen, MHC Class Ib Antigen, HLA-6.0, MHC-G, HLAG

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:100
Application Note IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0). Incubate the samples at RT (18-25°C) for 34 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human placenta


Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05%BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05%BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Gene Symbol HLA-G
Gene Full Name Major Histocompatibility Complex, Class I, G
Background HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]
Function Non-classical major histocompatibility class Ib molecule involved in immune regulatory processes at the maternal-fetal interface. In complex with B2M/beta-2 microglobulin binds a limited repertoire of nonamer self-peptides derived from intracellular proteins including histones and ribosomal proteins.Peptide-bound HLA-G-B2M complex acts as a ligand for inhibitory/activating KIR2DL4, LILRB1 and LILRB2 receptors on uterine immune cells to promote fetal development while maintaining maternal-fetal tolerance.Upon interaction with KIR2DL4 and LILRB1 receptors on decidual NK cells, it triggers NK cell senescence-associated secretory phenotype as a molecular switch to promote vascular remodeling and fetal growth in early pregnancy. [Uniprot]
Cellular Localization Cell membrane, Cell projection, Endoplasmic reticulum, Endosome, Membrane
Calculated MW 38 kDa
PTM N-glycosylated.1 Publication. Soluble HLA class I histocompatibility antigen, alpha chain G Produced by proteolytic cleavage at the cell surface (shedding) by matrix metalloproteinase MMP2. [Uniprot]

Images (1) Click the Picture to Zoom In

  • ARG67037 anti-HLA G antibody [SQab30313] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded human placenta stained with ARG67037 anti-HLA G antibody [SQab30313].