ARG45735
anti-HMGB3 / HMG4 antibody
anti-HMGB3 / HMG4 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes HMGB3 / HMG4 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | HMGB3 / HMG4 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to N-terminal region of human HMGB3 / HMG4. |
| Conjugation | Un-conjugated |
| Alternate Names | HMGB3; High Mobility Group Box 3; High mobility group protein 2a; HMG2A; HMG-2a; High mobility group protein B3; High mobility group protein 4; HMG4; HMG-4 |
Application Instructions
| Application Suggestion |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 23 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HMGB3 |
| Gene Full Name | High Mobility Group Box 3 |
| Background | HMGB3 belongs to the high mobility group (HMG) protein superfamily. Like HMG1 (MIM 163905) and HMG2 (MIM 163906), HMGB3 contains DNA-binding HMG box domains and is classified into the HMG box subfamily. Members of the HMG box subfamily are thought to play a fundamental role in DNA replication, nucleosome assembly and transcription (Wilke et al., 1997 [PubMed 9370291]; Nemeth et al., 2006 [PubMed 16945912]).[supplied by OMIM, Mar 2008] |
| Function | Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. Associates with chromatin and binds DNA with a preference for non-canonical DNA structures such as single-stranded DNA. Can bend DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters (By similarity). Proposed to be involved in the innate immune response to nucleic acids by acting as a cytoplasmic promiscuous immunogenic DNA/RNA sensor (By similarity). Negatively regulates B-cell and myeloid cell differentiation. In hematopoietic stem cells may regulate the balance between self-renewal and differentiation. Involved in negative regulation of canonical Wnt signaling (By similarity). [UniProt] |
| Cellular Localization | Chromosome; Cytoplasm; Nucleus. [UniProt] |
| Calculated MW | 23 kDa |
| PTM | Reduction/oxidation of cysteine residues Cys-23, Cys-45 and Cys-104 and a possible intramolecular disulfide bond involving Cys-23 and Cys-45 give rise to different redox forms with specific functional activities in various cellular compartments: 1- fully reduced HMGB3 (HMGB3C23hC45hC104h), 2- disulfide HMGB3 (HMGB3C23-C45C104h) and 3- sulfonyl HMGB3 (HMGB3C23soC45soC104so).. [UniProt] |
Images (7) Click the Picture to Zoom In
-
ARG45735 anti-HMGB3 / HMG4 antibody IHC-P image
Immunohistochemistry: Human placenta stained with ARG45735 anti-HMGB3 / HMG4 antibody at 2 μg/ml dilution.
-
ARG45735 anti-HMGB3 / HMG4 antibody ICC/IF image
Immunofluorescence: MCF-7 stained with ARG45735 anti-HMGB3 / HMG4 antibody at 5 μg/ml dilution.
-
ARG45735 anti-HMGB3 / HMG4 antibody WB image
Western blot: MCF-7 stained with ARG45735 anti-HMGB3 / HMG4 antibody at 0.5 μg/ml dilution.
-
ARG45735 anti-HMGB3 / HMG4 antibody FACS image
Flow Cytometry: HL-60 stained with ARG45735 anti-HMGB3 / HMG4 antibody at 1 µg/10^6 cells dilution.
-
ARG45735 anti-HMGB3 / HMG4 antibody IHC-P image
Immunohistochemistry: Rat intestine stained with ARG45735 anti-HMGB3 / HMG4 antibody at 2 μg/ml dilution.
-
ARG45735 anti-HMGB3 / HMG4 antibody IHC-P image
Immunohistochemistry: Mouse intestine stained with ARG45735 anti-HMGB3 / HMG4 antibody at 2 μg/ml dilution.
-
ARG45735 anti-HMGB3 / HMG4 antibody WB image
Western blot: Mouse liver stained with ARG45735 anti-HMGB3 / HMG4 antibody at 0.5 μg/ml dilution.
