ARG45610
anti-HMGCS2 antibody
anti-HMGCS2 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes HMGCS2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | HMGCS2 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human HMGCS2. |
| Conjugation | Un-conjugated |
| Alternate Names | HMGCS2; 3-Hydroxy-3-Methylglutaryl-CoA Synthase 2; 3-Hydroxy-3-Methylglutaryl-Coenzyme A Synthase 2 (Mitochondrial); 3-Hydroxy-3-Methylglutaryl-CoA Synthase 2 (Mitochondrial); Hydroxymethylglutaryl-CoA Synthase, Mitochondrial; HMG-CoA Synthase; EC 2.3.3.10; 3-Hydroxy-3-Methylglutaryl Coenzyme A Synthase; Testicular Tissue Protein Li 88 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 50 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P54868 Human Hydroxymethylglutaryl-CoA synthase, mitochondrial Swiss-port # P54869 Mouse Hydroxymethylglutaryl-CoA synthase, mitochondrial |
|---|---|
| Gene Symbol | HMGCS2 |
| Gene Full Name | 3-Hydroxy-3-Methylglutaryl-CoA Synthase 2 |
| Background | The protein encoded by this gene belongs to the HMG-CoA synthase family. It is a mitochondrial enzyme that catalyzes the first reaction of ketogenesis, a metabolic pathway that provides lipid-derived energy for various organs during times of carbohydrate deprivation, such as fasting. Mutations in this gene are associated with HMG-CoA synthase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009] |
| Function | Catalyzes the first irreversible step in ketogenesis, condensing acetyl-CoA to acetoacetyl-CoA to form HMG-CoA, which is converted by HMG-CoA reductase (HMGCR) into mevalonate. [UniProt] |
| Cellular Localization | Mitochondrion. [UniProt] |
| Calculated MW | 57 kDa |
| PTM | Acetylation; Phosphoprotein. [UniProt] |
Images (6) Click the Picture to Zoom In
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ARG45610 anti-HMGCS2 antibody IHC-P image
Immunohistochemistry: Human gastric cancer stained with ARG45610 anti-HMGCS2 antibody at 2 μg/ml dilution.
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ARG45610 anti-HMGCS2 antibody ICC/IF image
Immunofluorescence: CACO-2 stained with ARG45610 anti-HMGCS2 antibody at 5 μg/ml dilution.
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ARG45610 anti-HMGCS2 antibody WB image
Western blot: CACO-2 stained with ARG45610 anti-HMGCS2 antibody at 0.5 μg/ml dilution.
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ARG45610 anti-HMGCS2 antibody FACS image
Flow Cytometry: CACO-2 stained with ARG45610 anti-HMGCS2 antibody at 1 µg/10^6 cells dilution.
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ARG45610 anti-HMGCS2 antibody WB image
Western blot: Rat liver stained with ARG45610 anti-HMGCS2 antibody at 0.5 μg/ml dilution.
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ARG45610 anti-HMGCS2 antibody WB image
Western blot: Mouse liver stained with ARG45610 anti-HMGCS2 antibody at 0.5 μg/ml dilution.
