ARG10496

anti-HPV16 E7 antibody [716-D1]

anti-HPV16 E7 antibody [716-D1] for ELISA,Western blot and HPV

Microbiology and Infectious Disease antibody

Overview

Product Description Mouse Monoclonal antibody [716-D1] recognizes HPV16 E7
Tested Reactivity HPV
Tested Application ELISA, WB
Specificity The clone 716-D1 can detect both monomer and dimmer forms of HPV type 16 E7 protein and it is also equally suitable for capture of both HPV type 16 and 18 according WB result.
Host Mouse
Clonality Monoclonal
Clone 716-D1
Isotype IgG2a
Target Name HPV16 E7
Antigen Species Virus
Immunogen Balb/c mice immunized with HPV oncoprotein E7, type 16.
Epitope a.a. 36-43
Conjugation Un-conjugated
Full Name transforming protein

Application Instructions

Application Note ELISA: Recommended pair for sandwich immunoassay: ARG10496 (capture) and ARG11058 (detection)
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G affinity purified.
Buffer PBS (pH.2)) and 0.1 % Sodium azide
Preservative 0.1 % Sodium azide
Concentration 1.0-2.0 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 1489079 HPV E7

Gene Symbol E7
Gene Full Name transforming protein
Function E7 protein has both transforming and trans-activating activities. Disrupts the function of host retinoblastoma protein RB1/pRb, which is a key regulator of the cell cycle. Induces the disassembly of the E2F1 transcription factors from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Inactivation of the ability of RB1 to arrest the cell cycle is critical for cellular transformation, uncontrolled cellular growth and proliferation induced by viral infection. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. Interferes with histone deacetylation mediated by HDAC1 and HDAC2, leading to activation of transcription (By similarity). [UniProt]
Research Area Microbiology and Infectious Disease antibody

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  • ARG10496 anti-HPV16 E7 antibody [716-D1] WB image

    Western blot:15 µg of recominbinant protein stained with ARG10496 anti-HPV16 E7 antibody [716-D1].