ARG66339

anti-HRP antibody [SQab1887]

anti-HRP antibody [SQab1887] for Western blot,Flow cytometry,ICC/IF and Other

Overview

Product Description Recombinant Rabbit Monoclonal antibody [SQab1887] recognizes HRP
Tested Reactivity Other
Tested Application FACS, ICC/IF, WB
Host Rabbit
Clonality Monoclonal
Clone SQab1887
Isotype IgG
Target Name HRP
Antigen Species Others
Immunogen Full length native protein (purified) corresponding to HRP.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:50
ICC/IF1:200
WB1:5000 - 1:10000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 39 kDa

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (3) Click the Picture to Zoom In

  • ARG66339 anti-HRP antibody [SQab1887] ICC/IF image

    Immunofluorescence: HeLa cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66339 anti-HRP antibody [SQab1887] (green) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

  • ARG66339 anti-HRP antibody [SQab1887] FACS image

    Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66339 anti-HRP antibody [SQab1887] (red) at 1:50 dilution in 1x PBS/1% BSA for 60 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.

  • ARG66339 anti-HRP antibody [SQab1887] WB image

    Western blot: 20 ng of HRP protein stained with ARG66339 anti-HRP antibody [SQab1887] at 1:10000 dilution.