anti-Haptoglobin antibody

anti-Haptoglobin antibody for Flow cytometry,ICC/IF,Western blot and Human

Cell Biology and Cellular Response antibody
publication_link Publication1


Product Description

Rabbit Polyclonal antibody recognizes Haptoglobin

Tested Reactivity Hu
Tested Application FACS, ICC/IF, WB
Specificity The antibody was raised by the immunogen coresponding to a.a. 296-322 of human haptoglobin which expect recognizes to full length and Haptoglobin beta chain (40 kDa, 245 amino acids unglycosylated 35 kDa).
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Haptoglobin
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 296-322 of Human Haptoglobin.
Conjugation Un-conjugated
Alternate Names HPA1S; Haptoglobin; BP; HP2ALPHA2; Zonulin

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:50
ICC/IF1:10 - 1:50
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Purification with Protein A and immunogen peptide
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 3240 Human HP

Swiss-port # P00738 Human Haptoglobin

Gene Symbol HP
Gene Full Name haptoglobin
Background This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. The protein encoded also exhibits antimicrobial activity against bacteria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014]
Function As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway. [UniProt]
Research Area Cell Biology and Cellular Response antibody
Calculated MW 45 kDa

Images (4) Click the Picture to Zoom In

  • ARG55106 anti-Haptoglobin antibody WB image

    Western blot: 30 µg of HepG2 cell lysate stained with ARG55106 anti-Haptoglobin antibody at 1:1000 dilution.

  • ARG55106 anti-Haptoglobin antibody ICC/IF image

    Immunofluorescence: MDA-MB-435 cells stained with ARG55106 anti-Haptoglobin antibody (green). DAPI (blue) for nuclear staining.

  • ARG55106 anti-Haptoglobin antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human hepatocarcinoma stained with ARG55106 anti-Haptoglobin antibody.

  • ARG55106 anti-Haptoglobin antibody FACS image

    Flow Cytometry: MDA-MB435 cells stained with ARG55106 anti-Haptoglobin antibody (right histogram) or without primary antibody control (left histogram), followed by incubation with FITC labelled secondary antibody.

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Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Effect of haptoglobin on the treatment of chronic hepatitis B with interferon.

WB / Human

Lv Lin-Ling et al.
Experimental and Therapeutic Medicine.,  (2019)