anti-Helicobacter pylori CagA antibody [10E9]
anti-Helicobacter pylori CagA antibody [10E9] for ELISA,Western blot and Helicobacter pylori
Microbiology and Infectious Disease antibody
|Product Description||Mouse Monoclonal antibody [10E9] recognizes Helicobacter pylori CagA|
|Tested Reactivity||H. pylori|
|Tested Application||ELISA, WB|
|Specificity||Cross-reaction with other proteins has not been identified.|
|Target Name||Helicobacter pylori CagA|
|Immunogen||A highly immunogenic 32kD CagA fragment, recombinant|
|Application Note||ELISA: Clone 10E9 can be used as capture antibody in sandwich ELISA. Anti-CagA clone 10E9 coated wells detect recombinant CagA antigen in combination with HRP conjugated anti-CagA clone 5C6, clone 3C10 and clone 3C1. In addition, clone 10E9 selectively detected cell lysate of a CagA containing H. pylori strain when HRP conjugated anti-CagA clone 3C10 was used.
Western Blot: This clone detects the 32 kD band corresponding to the molecular weight of the recombinant CagA antigen (Immunogen).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Purification||Protein G affinity purified|
|Buffer||0.01M PBS (pH 7.0)|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Background||Helicobacter pylori (H. pylori), a spiral rod shaped gram-negative bacterium, is frequently found in the stomach. In infected populations, 10-20% may develop gastritis and gastric ulcer and 1-2% may develop cancer. The genome of H. pylori isolates from carriers with symptoms contains a 40kb pathogenicity island encoding H. pylori cytotoxin, cytotoxin associated gene A protein (CagA) and other virulence associated factors. CagA is used as a biomarker for virulent H. pylori strains.|
Helicobacter pylori antibodies; Helicobacter pylori ELISA Kits; Helicobacter pylori Duos / Panels; Anti-Mouse IgG secondary antibodies;
Tools for studying H. pylori diseases
|Research Area||Microbiology and Infectious Disease antibody|