ARG54779

anti-Histone H3 monomethyl (Arg17) antibody

anti-Histone H3 monomethyl (Arg17) antibody for Dot blot,ICC/IF,Western blot and Human

Gene Regulation antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Histone H3 monomethyl (Arg17)
Tested Reactivity Hu
Tested Application Dot, ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Histone H3
Antigen Species Human
Immunogen Synthetic methylated peptide around Arg17 of Human histone H3 (NP_003526.1)
Conjugation Un-conjugated
Alternate Names Histone H3/f; Histone H3.1; Histone H3/d; Histone H3/b; Histone H3/c; Histone H3/a; Histone H3/l; Histone H3/j; Histone H3/k; Histone H3/h; H3/A; H3FA; Histone H3/i

Application Instructions

Application Suggestion
Tested Application Dilution
DotAssay-dependent
ICC/IF1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8356 Human HIST1H3J

Swiss-port # P68431 Human Histone H3.1

Gene Symbol HIST1H3J
Gene Full Name histone cluster 1, H3j
Background Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]
Research Area Gene Regulation antibody
Calculated MW 15 kDa

Images (2) Click the Picture to Zoom In

  • ARG54779 anti-Histone H3 monomethyl (Arg17) antibody ICC/IF image

    Immunofluorescence: 293T cells stained with ARG54779 anti-Histone H3 monomethyl (Arg17) antibody.

  • ARG54779 anti-Histone H3 monomethyl (Arg17) antibody WB image

    Western blot: HeLa cell lysate and Recombinant Histone H3 protein expressed in E. coli (negative control) stained with ARG54779 anti-Histone H3 monomethyl (Arg17) antibody .