anti-Histone H4 monomethyl (Lys20) antibody

anti-Histone H4 monomethyl (Lys20) antibody for Dot blot,ICC/IF,Western blot and Human

Gene Regulation antibody


Product Description Rabbit Polyclonal antibody recognizes Histone H4 monomethyl (Lys20)
Tested Reactivity Hu
Tested Application Dot, ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Histone H4
Antigen Species Human
Immunogen Synthetic methylated peptide around Lys20 of Human histone H4 (NP_003539.1)
Conjugation Un-conjugated
Alternate Names H4/p; Histone H4

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa


Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 8370 Human HIST2H4A

Swiss-port # P62805 Human Histone H4

Gene Symbol HIST2H4A
Gene Full Name histone cluster 2, H4a
Background Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy. [provided by RefSeq, Jul 2008]
Research Area Gene Regulation antibody
Calculated MW 11 kDa

Images (2) Click the Picture to Zoom In

  • ARG54774 anti-Histone H4 monomethyl (Lys20) antibody ICC/IF image

    Immunofluorescence: 293T cells stained with ARG54774 anti-Histone H4 monomethyl (Lys20) antibody.

  • ARG54774 anti-Histone H4 monomethyl (Lys20) antibody WB image

    Western blot: HeLa cell lysate and Recombinant Histone H4 protein expressed in E. coli (negative control) stained with ARG54774 anti-Histone H4 monomethyl (Lys20) antibody.