ARG54443
anti-Hsc 70 antibody [1B5]
anti-Hsc 70 antibody [1B5] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Bovine,Chicken,Dog,Guinea pig,Hamster,Monkey,Pig,Rabbit,Sheep
Signaling Transduction antibody
Overview
| Product Description | Rat Monoclonal antibody [1B5] recognizes Hsc 70 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Bov, Chk, Dog, Gpig, Hm, Mk, Pig, Rb, Sheep |
| Tested Application | ICC/IF, IHC-P, IP, WB |
| Specificity | This antibody detects a 73 kDa protein on HeLa Heat Shocked Cell Lysate SDS-PAGE immunoblots, corresponding to the molecular weight of Hsc70. This antibody reacts with human, monkey, mouse, hamster, guinea pig, rat, rabbit, bovine, shep, pig, dog, and chicken Hsc70. |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | 1B5 |
| Isotype | IgG2a |
| Target Name | Hsc 70 |
| Antigen Species | Hamster |
| Immunogen | Hsc70 purified from sodium arsenite treated heat-resistant variants of Chinese hamster cells. |
| Conjugation | Un-conjugated |
| Alternate Names | NIP71; HEL-33; HSPA10; LAP-1; HSC70; HSC71; LAP1; Lipopolysaccharide-associated protein 1; Heat shock 70 kDa protein 8; HSC54; HEL-S-72p; Heat shock cognate 71 kDa protein; HSP71; HSP73; LPS-associated protein 1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein G Purified |
| Buffer | PBS (pH 7.2) and 0.1mM PMSF in 50% Glycerol |
| Stabilizer | 0.1mM PMSF in 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Hspa8 |
| Gene Full Name | heat shock 70kDa protein 8 |
| Background | This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011] |
| Function | Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex (By similarity). Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion. Participates in the ER-associated degradation (ERAD) quality control pathway in conjunction with J domain-containing co-chaperones and the E3 ligase CHIP (By similarity). [UniProt] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 71 kDa |
| PTM | Acetylated. ISGylated. Trimethylation at Lys-561 reduces fibrillar SNCA binding. |
Clone References
A cytosolic chaperone complexes with dynamic membrane J-proteins and mobilizes a nonenveloped virus out of the endoplasmic reticulum.
IP /
MiR-17-5p impairs trafficking of H-ERG K+ channel protein by targeting multiple er stress-related chaperones during chronic oxidative stress.
WB / Rat, Human
scyllo-Inositol promotes robust mutant Huntingtin protein degradation.
Mutation in integrin-linked kinase (ILK(R211A)) and heat-shock protein 70 comprise a broadly cardioprotective complex.
WB / Human
Proteasome inhibition alleviates SNARE-dependent neurodegeneration.
WB / Mouse
Overexpression of ST6GalNAcV, a ganglioside-specific alpha2,6-sialyltransferase, inhibits glioma growth in vivo.
IP / Human
Photoreceptor IFT complexes containing chaperones, guanylyl cyclase 1 and rhodopsin.
IP / Cow
Phosphorylation-dependent interaction of tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein (YWHA) with PADI6 following oocyte maturation in mice.
IP / Human
