ARG22206

anti-Hsc 70 antibody [1F2-H5]

anti-Hsc 70 antibody [1F2-H5] for Western blot,Immunohistochemistry,ICC/IF,Immunoprecipitation,ELISA and Human,Mouse,Rat

Overview

Product Description Mouse Monoclonal antibody [1F2-H5] recognizes Hsc 70
Tested Reactivity Hu, Ms, Rat
Tested Application ELISA, ICC/IF, IHC, IP, WB
Specificity Detects ~73kDa. Does not cross react with HSP70.
Host Mouse
Clonality Monoclonal
Clone 1F2-H5
Isotype IgG2a, kappa
Target Name Hsc 70
Antigen Species Human
Immunogen Full length Human HSC70
Conjugation Un-conjugated
Alternate Names NIP71; HEL-33; HSPA10; LAP-1; HSC70; HSC71; LAP1; Lipopolysaccharide-associated protein 1; Heat shock 70 kDa protein 8; HSC54; HEL-S-72p; Heat shock cognate 71 kDa protein; HSP71; HSP73; LPS-associated protein 1

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
ICC/IF1:100
IHCAssay-dependent
IPAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 15481 Mouse HSPA8

GeneID: 24468 Rat HSPA8

GeneID: 3312 Human HSPA8

Gene Symbol HSPA8
Gene Full Name heat shock 70kDa protein 8
Background This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
Function Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Participates in the ER-associated degradation (ERAD) quality control pathway in conjunction with J domain-containing co-chaperones and the E3 ligase CHIP. [UniProt]
Cellular Localization Cytoplasm, Melanosome
Calculated MW 71 kDa
PTM Acetylated.
ISGylated.
Trimethylation at Lys-561 reduces fibrillar SNCA binding.

Images (3) Click the Picture to Zoom In

  • ARG22206 anti-Hsc 70 antibody [1F2-H5] ICC/IF image

    Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22206 anti-Hsc 70 antibody [1F2-H5] (green) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.

  • ARG22206 anti-Hsc 70 antibody [1F2-H5] WB image

    Western blot: 15 µg of Human cell lysates stained with ARG22206 anti-Hsc 70 antibody [1F2-H5] at 1:1000 dilution (2 hours at RT).

  • ARG22206 anti-Hsc 70 antibody [1F2-H5] ICC/IF image

    Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22206 anti-Hsc 70 antibody [1F2-H5] (yellow) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 20x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.