ARG22218

anti-Hsp 27 antibody [5D12-A12]

anti-Hsp 27 antibody [5D12-A12] for Western blot,Immunohistochemistry,ICC/IF,Immunoprecipitation,ELISA and Human

Overview

Product Description Mouse Monoclonal antibody [5D12-A12] recognizes Hsp 27
Tested Reactivity Hu
Tested Application ELISA, ICC/IF, IHC, IP, WB
Specificity Detects ~27kDa. Has no cross-reactivity to Alpha B crystallin. Very limited cross-reactivity to other species.
Host Mouse
Clonality Monoclonal
Clone 5D12-A12
Isotype IgG2b, kappa
Target Name Hsp 27
Antigen Species Human
Immunogen Human HSP27
Conjugation Un-conjugated
Alternate Names HSP 27; Heat shock 27 kDa protein; HMN2B; HS.76067; SRP27; HEL-S-102; HspB1; CMT2F; 28 kDa heat shock protein; HSP27; Heat shock protein beta-1; Hsp25; Estrogen-regulated 24 kDa protein; Stress-responsive protein 27; HSP28

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
ICC/IF1:100
IHCAssay-dependent
IPAssay-dependent
WB1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3315 Human HSPB1

Swiss-port # P04792 Human Heat shock protein beta-1

Gene Symbol HSPB1
Gene Full Name heat shock 27kDa protein 1
Background The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008]
Function Involved in stress resistance and actin organization. [UniProt]
Cellular Localization Cytoplasm, cytoskeleton, Nucleus, spindle
Calculated MW 23 kDa
PTM Phosphorylated upon exposure to protein kinase C activators and heat shock (PubMed:8325890). Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress dissociates HSPB1 from large small heat-shock protein (sHsps) oligomers and impairs its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement (PubMed:1332886, PubMed:8093612, PubMed:19166925).

Images (3) Click the Picture to Zoom In

  • ARG22218 anti-Hsp 27 antibody [5D12-A12] ICC/IF image

    Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22218 anti-Hsp 27 antibody [5D12-A12] (green) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.

  • ARG22218 anti-Hsp 27 antibody [5D12-A12] WB image

    Western blot: Human cell lysates stained with ARG22218 anti-Hsp 27 antibody [5D12-A12] at 1:1000 dilution.

  • ARG22218 anti-Hsp 27 antibody [5D12-A12] ICC/IF image

    Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22218 anti-Hsp 27 antibody [5D12-A12] (green) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 20x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.